Mouse anti-Human CRY1 Monoclonal Antibody | anti-CRY1 antibody

Mouse Monoclonal [clone 4H4-1C4] (IgG1,k) to Human CRY1

Cat. Num. AAA246144

• Gene Names: CRY1; PHLL1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: CRY1; Monoclonal Antibody; Mouse Monoclonal [clone 4H4-1C4] (IgG1; k) to Human CRY1; Anti-CRY1 Antibody (clone 4H4-1C4) IHC-plus; Chryptochrome 1; Cryptochrome-1; Photolyase homolog 1; PHLL1; Human CRY1; anti-CRY1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4H4-1C4
• Specificity: Human CRY1
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 0.4 mg/ml (varies by lot)
• Sequence Length: 586

• Applicable Applications for anti-CRY1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (10 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: CRY1 (AAH30519, 1 a.a. ~ 587 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: CRY1 antibody was raised against cRY1 (AAH30519, 1 a.a. ~ 587 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-CRY1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to CRY1 on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(CRY1 monoclonal antibody, clone 4H4-1C4 Western blot of CRY1 expression in HeLa nuclear extract..)

NCBI and Uniprot Product Information

• NCBI GI #: 4758072
• NCBI GeneID: 1407
• NCBI Accession #: NP_004066.1
• NCBI GenBank Nucleotide #: NM_004075.4
• UniProt Accession #: Q16526
• Molecular Weight: 66,395 Da
• NCBI Official Full Name: cryptochrome-1
• NCBI Official Synonym Full Names: cryptochrome circadian clock 1
• NCBI Official Symbol: CRY1
• NCBI Official Synonym Symbols: PHLL1
• NCBI Protein Information: cryptochrome-1; cryptochrome 1 (photolyase-like)
• UniProt Protein Name: Cryptochrome-1
• Protein Family: Cryptochrome
• UniProt Gene Name: CRY1
• UniProt Synonym Gene Names: PHLL1
• UniProt Entry Name: CRY1_HUMAN

NCBI Description

This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Loss of the related gene in mouse results in a shortened circadian cycle in complete darkness. [provided by RefSeq, Jan 2014]

Uniprot Description

CRY1: Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK|NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Interacts directly with TIMELESS and the PER proteins. Interacts directly with PER1 and PER2 C-terminal domains. Interaction with PER2 inhibits its ubiquitination and vice versa. Binds MAPK. Interacts with FBXL21. Interacts with FBXL3. Expression is regulated by light and circadian rhythms. Peak expression in the suprachiasma nucleus (SCN) and eye at the day/night transition (CT12). Levels decrease with ARNTL-CLOCK inhibition as part of the autoregulatory feedback loop. Belongs to the DNA photolyase class-1 family.

Protein type: DNA-binding; Transcription, coactivator/corepressor; Lyase

Chromosomal Location of Human Ortholog: 12q23-q24.1

Cellular Component: mitochondrion; nucleus

Molecular Function: ubiquitin binding; DNA (6-4) photolyase activity; histone deacetylase binding; nucleotide binding; protein kinase binding; transcription factor binding; phosphatase binding; protein binding; DNA binding; double-stranded DNA binding; deoxyribodipyrimidine photo-lyase activity; blue light photoreceptor activity; nuclear hormone receptor binding

Biological Process: circadian rhythm; response to glucagon stimulus; DNA damage induced protein phosphorylation; transcription, DNA-dependent; negative regulation of G-protein coupled receptor protein signaling pathway; negative regulation of transcription from RNA polymerase II promoter; regulation of circadian rhythm; glucose homeostasis; DNA repair; protein-chromophore linkage; entrainment of circadian clock by photoperiod; response to insulin stimulus; gluconeogenesis; blue light signaling pathway; sequestering of lipid; negative regulation of protein ubiquitination; negative regulation of circadian rhythm; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent