Mouse CrkL Monoclonal Antibody | anti-CRKL antibody

CrkL (Crk-like protein, v-crk sarcoma virus CT10 oncogene homolog (avian)-like)

Cat. Num. AAA604626

• Gene Names: CRKL; CRKL
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G immunoaffinity chromatography.
• Synonyms: CrkL; Monoclonal Antibody; CrkL (Crk-like protein; v-crk sarcoma virus CT10 oncogene homolog (avian)-like); Anti -CrkL (Crk-like protein; anti-CRKL antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9H105
• Specificity: Detects endogenous human, mouse and rat CrkL at 39kD using Western blot.
• Purity/Purification: Affinity Purified; Purified by Protein G immunoaffinity chromatography.
• Form/Format: Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml.

• Applicable Applications for anti-CRKL antibody: Western Blot (WB)
• Application Notes: Dilution: Western Blot:1ug/ml. Lysates from human K562, A431 and Jurkat cells were resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml mouse anti-CrkL
• Immunogen: Purified, E. coli-derived full length recombinant human CrkL (rhCrkL; aa 1-303; Accession# P46109).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CRKL antibody

CrkL is a widely-expressed 39kD adaptor protein that belongs to the Crk protein family and is

Product Categories/Family for anti-CRKL antibody

Antibodies; Abs to Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 4885153
• NCBI GeneID: 1399
• NCBI Accession #: NP_005198.1
• NCBI GenBank Nucleotide #: NM_005207.3
• UniProt Accession #: P46109; A8KA44; D3DX35
• Molecular Weight: 33,777 Da
• NCBI Official Full Name: crk-like protein
• NCBI Official Synonym Full Names: v-crk sarcoma virus CT10 oncogene homolog (avian)-like
• NCBI Official Symbol: CRKL
• NCBI Official Synonym Symbols: CRKL
• NCBI Protein Information: crk-like protein; OTTHUMP00000198538; OTTHUMP00000198539
• UniProt Protein Name: Crk-like protein
• Protein Family: Crk-like protein
• UniProt Gene Name: CRKL
• UniProt Entry Name: CRKL_HUMAN

NCBI Description

This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

Uniprot Description

CRKL: an oncogenic adaptor protein with an SH2-SH3-SH3 domain structure. Plays a specific role in integrin-induced migration as a downstream mediator of Src. Activates the RAS and Jnk signaling pathways. Transforms fibroblasts in a RAS-dependent fashion. A substrate of BCR-ABL and plays a role in fibroblast transformation by BCR-ABL.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: cytosol; endosome

Molecular Function: signal transducer activity; protein binding; SH3/SH2 adaptor activity

Biological Process: anterior/posterior pattern formation; blood vessel development; organ morphogenesis; thymus development; activation of MAPKK activity; nerve growth factor receptor signaling pathway; heart development; Ras protein signal transduction; JNK cascade; parathyroid gland development