Western Blot (WB)
(NIH/3T3 cells were subjected to SDS PAGE followed by western blot with MBS768204 (CREST antibody) at dilution of 1:3000)
Mouse anti-Human CREST Monoclonal Antibody | anti-CREST antibody
CREST Mouse Monoclonal
Reactivity
Human
Applications
ELISA, Western Blot
Purity
>=95% as determined by SDS-PAGEProtein A+G purification
Synonyms
CREST; Monoclonal Antibody; CREST Mouse Monoclonal; KIAA0693; LP2261; SS18 like protein 1; SS18L1; SYT homolog 1; anti-CREST antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
Human, Mouse, Rat; other species are not tested. Please decide the specificity by homology
Purity/Purification
>=95% as determined by SDS-PAGE
Protein A+G purification
Protein A+G purification
Form/Format
Liquid
Applicable Applications for anti-CREST antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB : 1:1000-1:6000
Immunogen
Synovial sarcoma translocation gene on chromosome 18-like 1
Preparation and Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)
Western Blot (WB)
(NIH/3T3 cells were subjected to SDS PAGE followed by western blot with MBS768204 (CREST antibody) at dilution of 1:3000)
Western Blot (WB)
(NIH/3T3 cells were subjected to SDS PAGE followed by western blot with MBS768204 (CREST antibody) at dilution of 1:3000)
Related Product Information for anti-CREST antibody
Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity).
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The CREST (Catalog #AAA768204) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CREST Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CREST can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB : 1:1000-1:6000. Researchers should empirically determine the suitability of the CREST for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CREST, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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