Mouse Creatine Phosphokinase-BB (CK-BB) Monoclonal Antibody | anti-CKB antibody

Creatine Phosphokinase-BB (CK-BB) Mouse Monoclonal Antibody

Cat. Num. AAA439347

• Gene Names: CKB; BCK; B-CK; CKBB; HEL-211; HEL-S-29
• Reactivity: Human.; Predicted to react with Chimpanzee, Rhesus Monkey, Dog, Cow, Mouse, Rat, Chicken, Zebrafish, and Frog.; Others not tested
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: Creatine Phosphokinase-BB (CK-BB); Monoclonal Antibody; Creatine Phosphokinase-BB (CK-BB) Mouse Monoclonal Antibody; B-CK; BB-CK; creatine kinase-B; Creatine kinase B chain; CKBB creatine kinase B-type; creatine kinase; brain; CKBB; Brain creatine kinase; Creatine kinase B-type; Creatine Kinase BB Isoenzyme; Creatine kinase brain; Creatine phosphokinase BB; anti-CKB antibody

• Host: Mouse
• Reactivity: Human.; Predicted to react with Chimpanzee, Rhesus Monkey, Dog, Cow, Mouse, Rat, Chicken, Zebrafish, and Frog.; Others not tested
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 2ba6
• Specificity: Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteins
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 381

• Applicable Applications for anti-CKB antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Immunohistochemistry (Frozen Only) (0.5-1.0ug/ml for 30 minutes at RT)
• Cellular Localization: Cytoplasmic
• Immunogen: Human CKBB protein
• Hu-Chromosome Location: 14q32
• Positive Control: Cerebellum
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Mariman, E.C., et al. 1987. Structure and expression of the human creatine kinase B gene. Genomics 1: 126-137. Mariman, E.C., et al. 1989. Complete nucleotide sequence of the human creatine kinase B gene. Nucleic Acids Res. 17: 6385

NCBI and Uniprot Product Information

• NCBI GI #: 21536286
• NCBI GeneID: 1152
• NCBI Accession #: NP_001814.2
• NCBI GenBank Nucleotide #: NM_001823.4
• UniProt Accession #: P12277; Q2LE07; Q6FG40; Q9UC66; A8K236; B2R5R4
• Molecular Weight: 43kDa (Monomer); 86kDa (Dimer)
• NCBI Official Full Name: creatine kinase B-type
• NCBI Official Synonym Full Names: creatine kinase B
• NCBI Official Symbol: CKB
• NCBI Official Synonym Symbols: BCK; B-CK; CKBB; HEL-211; HEL-S-29
• NCBI Protein Information: creatine kinase B-type
• UniProt Protein Name: Creatine kinase B-type
• Protein Family: Creatine kinase
• UniProt Gene Name: CKB
• UniProt Synonym Gene Names: CKBB
• UniProt Entry Name: KCRB_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

CKB: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: Amino Acid Metabolism - arginine and proline; EC 2.7.3.2; Kinase, other

Chromosomal Location of Human Ortholog: 14q32

Cellular Component: cytoplasm; cytosol; extracellular space

Molecular Function: creatine kinase activity; protein binding; ubiquitin protein ligase binding

Biological Process: creatine metabolic process; substantia nigra development