Western Blot (WB)
(Western Blot analysis (reducing conditions) of CRACC in mouse thymus using MBS607488 (2ug/ml). IgG (HRP) anti-rat secondary antibody was used.)
Rat anti-Mouse CRACC Monoclonal Antibody | anti-SLAMF7 antibody
CRACC (SLAMF7, SLAM family member 7, 19A, CSI, CD2-like receptor-activating cytotoxic cells, CD2 subset 1, CD319, CS1, Membrane protein FOAP-12, Novel Ly9, Protein 19A, UNQ576/PRO1138)
Gene Names
SLAMF7; 19A; CS1; CD319; CRACC; SLAMF7
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein G immunoaffinity chromatography.
Synonyms
CRACC; Monoclonal Antibody; CRACC (SLAMF7; SLAM family member 7; 19A; CSI; CD2-like receptor-activating cytotoxic cells; CD2 subset 1; CD319; CS1; Membrane protein FOAP-12; Novel Ly9; Protein 19A; UNQ576/PRO1138); Anti -CRACC (SLAMF7; anti-SLAMF7 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
9H101
Specificity
Detects rmCRACC in direct ELISAs and Western blots. In direct ELISAs, this antibody shows no cross-reactivity with rhCRACC, rmCD229, rmCD84, or rmNTB-A.
Purity/Purification
Affinity Purified
Purified by Protein G immunoaffinity chromatography.
Purified by Protein G immunoaffinity chromatography.
Form/Format
Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml.
Applicable Applications for anti-SLAMF7 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA, Western Blot.
Dilution: Western Blot: 2ug/ml. Lysates were prepared from mouse thymus extract in reducing sample
buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to an Immobilon-P membrane. Use of this antibody under non-reducing conditions is not recommended. The blot was developed with 2ug/ml antibody and chemiluminescent detection substrate.
ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect mouse CRACC. The detection limit for rmCRACC is approximately 1ng/well.
Dilution: Western Blot: 2ug/ml. Lysates were prepared from mouse thymus extract in reducing sample
buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to an Immobilon-P membrane. Use of this antibody under non-reducing conditions is not recommended. The blot was developed with 2ug/ml antibody and chemiluminescent detection substrate.
ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect mouse CRACC. The detection limit for rmCRACC is approximately 1ng/well.
Immunogen
Purified, CHO-derived, recombinant mouse CRACC (rmCRACC; aa 22 - 224; Accession# Q8BHK6).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western Blot analysis (reducing conditions) of CRACC in mouse thymus using MBS607488 (2ug/ml). IgG (HRP) anti-rat secondary antibody was used.)
Western Blot (WB)
(Western Blot analysis (reducing conditions) of CRACC in mouse thymus using MBS607488 (2ug/ml). IgG (HRP) anti-rat secondary antibody was used.)
Related Product Information for anti-SLAMF7 antibody
CRACC, also known as CSI (CD2 subset I), is a type I transmembrane protein belonging to
Product Categories/Family for anti-SLAMF7 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
37,421 Da
NCBI Official Full Name
CD2-like receptor activating cytotoxic cells
NCBI Official Synonym Full Names
SLAM family member 7
NCBI Official Symbol
SLAMF7
NCBI Official Synonym Symbols
19A; CS1; CD319; CRACC; SLAMF7
NCBI Protein Information
SLAM family member 7; protein 19A; CD2 subset 1; 19A24 protein; membrane protein FOAP-12; CD2-like receptor activating cytotoxic cells; CD2-like receptor-activating cytotoxic cells; novel LY9 (lymphocyte antigen 9) like protein
UniProt Protein Name
SLAM family member 7
Protein Family
UniProt Gene Name
SLAMF7
UniProt Synonym Gene Names
CS1
UniProt Entry Name
SLAF7_HUMAN
Uniprot Description
SLAMF7: Isoform 1 mediates NK cell activation through a SH2D1A- independent extracellular signal-regulated ERK-mediated pathway. May play a role in lymphocyte adhesion. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Immunoglobulin superfamily; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q23.1-q24.1
Cellular Component: integral to membrane
Molecular Function: receptor activity
Biological Process: natural killer cell mediated cytotoxicity; natural killer cell activation; cell adhesion
Research Articles on SLAMF7
1. altered expression of splice variants of CS1 and 2B4 that mediate differential signalling in PBMC from patients with SLE.
2. Data show that pair-wise ligations of 2B4 with DNAM-1 and/or NKG2D lead to increased effector functions of primary CD4(+)CD28(-) T cells to suboptimal levels of anti-CD3 stimulation.
3. CS1 was expressed at adhesion-promoting uropod membranes of polarized Multiple Myeloma cells, andis required for MM cell adhesion to bone marrow stromal cells
4. HuLuc63 eliminates myeloma cells, at least in part, via NK-mediated ADCC and shows the therapeutic potential of targeting CS1 with HuLuc63 for the treatment of multiple myeloma.
5. Observational study of gene-disease association. (HuGE Navigator)
6. 2B4, NTB-A and CRACC have roles in the regulation of Natural Killer cell function [review]
7. CS1 may play a role in the regulation of B lymphocyte proliferation during immune responses
8. CS1-L and CS1-S may differentially regulate human NK cell functions
9. blocking the engagement of 2B4, NTB-A and CRACC has no effect on the proliferation or development of the cytotoxic potential of NK cells but triggering by their physiological ligands on MHC class I-negative target cells induces potent NK cell cytotoxicity
2. Data show that pair-wise ligations of 2B4 with DNAM-1 and/or NKG2D lead to increased effector functions of primary CD4(+)CD28(-) T cells to suboptimal levels of anti-CD3 stimulation.
3. CS1 was expressed at adhesion-promoting uropod membranes of polarized Multiple Myeloma cells, andis required for MM cell adhesion to bone marrow stromal cells
4. HuLuc63 eliminates myeloma cells, at least in part, via NK-mediated ADCC and shows the therapeutic potential of targeting CS1 with HuLuc63 for the treatment of multiple myeloma.
5. Observational study of gene-disease association. (HuGE Navigator)
6. 2B4, NTB-A and CRACC have roles in the regulation of Natural Killer cell function [review]
7. CS1 may play a role in the regulation of B lymphocyte proliferation during immune responses
8. CS1-L and CS1-S may differentially regulate human NK cell functions
9. blocking the engagement of 2B4, NTB-A and CRACC has no effect on the proliferation or development of the cytotoxic potential of NK cells but triggering by their physiological ligands on MHC class I-negative target cells induces potent NK cell cytotoxicity
Similar Products
Product Notes
The SLAMF7 slamf7 (Catalog #AAA607488) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The CRACC (SLAMF7, SLAM family member 7, 19A, CSI, CD2-like receptor-activating cytotoxic cells, CD2 subset 1, CD319, CS1, Membrane protein FOAP-12, Novel Ly9, Protein 19A, UNQ576/PRO1138) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CRACC can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA, Western Blot. Dilution: Western Blot: 2ug/ml. Lysates were prepared from mouse thymus extract in reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to an Immobilon-P membrane. Use of this antibody under non-reducing conditions is not recommended. The blot was developed with 2ug/ml antibody and chemiluminescent detection substrate. ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect mouse CRACC. The detection limit for rmCRACC is approximately 1ng/well. Researchers should empirically determine the suitability of the SLAMF7 slamf7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CRACC, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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