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Western Blot (WB) (Western blot analysis of SARS-COV-2 N Protein using SARS-COV-2 N Protein Rabbit pAb (MBS9141939) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

Rabbit COVID 19 Nucleoprotein Coronavirus Monoclonal Antibody | anti-COVID-19 antibody

2019-nCoV N Protein Rabbit mAb

Reactivity
Reactivity SARS-CoV-2 Nucleoprotein
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Synonyms
COVID 19 Nucleoprotein Coronavirus; Monoclonal Antibody; 2019-nCoV N Protein Rabbit mAb; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Nucleoprotein; anti-COVID-19 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Reactivity SARS-CoV-2 Nucleoprotein
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Applicable Applications for anti-COVID-19 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:1000-1:5000
ELISA: 1:1000-1:5000
FC/FACS: 1:50-1:200
IHC: 1:50-1:200
IF: 1:50-1:200
IP: 1:50-1:200
Category
Primary Antibody
Immunogen
Recombinant fusion protein of SARS-COV-2 Nucleoprotein.
Preparation and Storage
Store at 4°C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of SARS-COV-2 N Protein using SARS-COV-2 N Protein Rabbit pAb (MBS9141939) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

Western Blot (WB) (Western blot analysis of SARS-COV-2 N Protein using SARS-COV-2 N Protein Rabbit pAb (MBS9141939) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Related Product Information for anti-COVID-19 antibody
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a test tool.

NCBI and Uniprot Product Information

NCBI GeneID
UniProt Accession #

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Product Notes

The COVID-19 (Catalog #AAA9141939) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The 2019-nCoV N Protein Rabbit mAb reacts with Reactivity SARS-CoV-2 Nucleoprotein and may cross-react with other species as described in the data sheet. AAA Biotech's COVID 19 Nucleoprotein Coronavirus can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:1000-1:5000 ELISA: 1:1000-1:5000 FC/FACS: 1:50-1:200 IHC: 1:50-1:200 IF: 1:50-1:200 IP: 1:50-1:200. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Nucleoprotein Coronavirus, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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