Rat Connexin 32 Monoclonal Antibody | anti-GJB1 antibody

Connexin 32 (Gap Junction Protein)

Cat. Num. AAA4380385

• Gene Names: Gjb1; Cx32; Cnx32; connexin-32
• Reactivity: Mouse, Rat. Others not known.
• Applications: Immunofluorescence, Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry
• Synonyms: Connexin 32; Monoclonal Antibody; Connexin 32 (Gap Junction Protein); Charcot Marie Tooth neuropathy X linked; CMTX; CMTX1; Connexin-32; Cx32; GAP junction 28kDa liver protein; Gap junction beta-1 protein; Gap junction protein beta 1 32kD; GJB1; anti-GJB1 antibody

• Host: Rat
• Reactivity: Mouse, Rat. Others not known.
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: R5.21C
• Specificity: This Ab recognizes a protein of 27-32kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 283

• Applicable Applications for anti-GJB1 antibody: Immunofluorescence (IF), Flow Cytometry (FC/FACS), Western Blot (WB), Immunohistochemistry (IHC) Formalin
• Application Notes: IF: 1-2ug/ml; FC/FACS: 0.5-1ug/million cells; WB: 0.5-1ug/ml; IHC-F: 0.5-1.0ug/ml for 30 minutes at RT.
• Immunogen: Mouse liver DOC-JR-plasma membranes
• Cellular Localization: Cytoplasmic and Cell Surface
• Positive Control: MCF-7 Cells. Pancreas, Liver, Kidney, Stomach or Tonsil
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

J. Cell Biol. 103, 755-766. Biol. Reprod. 49, 1277-1287. J. Cell Sci. 116, 3315-3325.

NCBI and Uniprot Product Information

• NCBI GI #: 699114748
• NCBI GeneID: 14618
• NCBI Accession #: NP_001289425.1
• NCBI GenBank Nucleotide #: NM_001302496.1
• UniProt Accession #: P28230
• Molecular Weight: 27-32kDa
• NCBI Official Full Name: gap junction beta-1 protein
• NCBI Official Synonym Full Names: gap junction protein, beta 1
• NCBI Official Symbol: Gjb1
• NCBI Official Synonym Symbols: Cx32; Cnx32; connexin-32
• NCBI Protein Information: gap junction beta-1 protein
• UniProt Protein Name: Gap junction beta-1 protein
• Protein Family: Gap junction beta-1 protein
• UniProt Gene Name: Gjb1
• UniProt Synonym Gene Names: Cxn-32; Cx32

NCBI Description

This gene is a member of the gap junction protein (connexin) family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of ions and small molecules between cells. Mutations in a similar gene in human cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Oct 2014]

Uniprot Description

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.