Mouse Connexin 32 Monoclonal Antibody | anti-GJB1 antibody

Connexin 32

Cat. Num. AAA600432

• Gene Names: GJB1; CMTX; CX32; CMTX1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Puri ed from mouse ascites by immunoaffinity chromatography.
• Synonyms: Connexin 32; Monoclonal Antibody; Anti -Connexin 32; anti-GJB1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3G103
• Specificity: Recognizes rat Connexin-32. Species Crossreactivity: Human and mouse.
• Purity/Purification: Affinity Purified; Puri ed from mouse ascites by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

• Applicable Applications for anti-GJB1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 0.1-1.0ug/ml; Western Blot: 1ug/ml. Reactivity has been con rmed by Western blot analysis of extracts derived from mouse liver, rat liver, rat primary hepatocytes and rat spinal cord.; Immunohistochemistry (frozen sections): 1-2ug/ml; Immuno uorescence: 5-10ug/ml
• Immunogen: Synthetic peptide corresponding to a segment of the cytoplasmic loop of rat Connexin-32.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-GJB1 antibody

Intracellular communication mediated by gap junctions plays an important role in a variety of cellular processes including: homeostasis, morphogenesis, cell differentiation, and growth control. Gap junctions are transmembrane channels that serve to directly link neighboring cells by mediating the exchange of low-molecular weight metabolites, ions, and second messengers. Gap junctions are formed by the interaction of connexons or hemichannels on adjacent cells. The connexon itself is composed of a hexameric assembly of proteins referred to as connexins. Connexins are highly homologous proteins encoded by a multigene family. The connexins exhibit similar structural features, which include a cytoplasmic amino terminal region, four transmembrane domains, two extracellular loops, and a carboxy-terminal cytoplasmic tail of varying length. Comparison of the amino acid sequences of the various connexin family members indicate that the two areas of greatest divergence amongst the connexin family members are the intracellular loop connecting the second and third transmembrane segments and the carboxy-terminal tail (1, 3, 6). These domains are, therefore, thought to mediate connexin-type speci c properties including: phosphorylation, responses to gating stimuli, as well as assembly and membrane turnover. Modulation of gap junctional communication can be achieved by multiple mechanisms and can occur very rapidly or over a period of several hours. These mechanisms include alterations in transcription, translation, stability, postranslational processing (especially phosphorylation), gating, and insertion or removal from the plasma membrane. Interestingly, reduction or alterations in the levels or types of connexin expressed in a given cell type has been found to correlate with tumor progression and metastasis (5).

Product Categories/Family for anti-GJB1 antibody

Antibodies; Abs to Connexin; Junction Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 974141
• NCBI GeneID: 2705
• UniProt Accession #: P08034; Q5U0S4; B2R8R2; D3DVV2
• Molecular Weight: 32,025 Da
• NCBI Official Full Name: connexin 32
• NCBI Official Synonym Full Names: gap junction protein, beta 1, 32kDa
• NCBI Official Symbol: GJB1
• NCBI Official Synonym Symbols: CMTX; CX32; CMTX1
• NCBI Protein Information: gap junction beta-1 protein; connexin 32; connexin-32; OTTHUMP00000023502; OTTHUMP00000023503; OTTHUMP00000023504; GAP junction 28 kDa liver protein
• UniProt Protein Name: Gap junction beta-1 protein
• Protein Family: Gap junction beta-1 protein
• UniProt Gene Name: GJB1
• UniProt Synonym Gene Names: CX32
• UniProt Entry Name: CXB1_HUMAN

NCBI Description

This gene encodes a member of the gap junction protein family. The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Uniprot Description

GJB1: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Defects in GJB1 are the cause of Charcot-Marie-Tooth disease X-linked type 1 (CMTX1); also designated CMT- X. CMTX1 is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot- Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies characterized by severely reduced motor nerve conduction velocities (NCVs) (less than 38m/s) and segmental demyelination and remyelination, and primary peripheral axonal neuropathies characterized by normal or mildly reduced NCVs and chronic axonal degeneration and regeneration on nerve biopsy. CMTX1 has both demyelinating and axonal features. Central nervous system involvement may occur. Defects in GJB1 may contribute to the phenotype of Dejerine-Sottas syndrome (DSS); also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie-Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine- Sottas syndrome. Belongs to the connexin family. Beta-type (group I) subfamily.

Protein type: Membrane protein, integral; Channel, misc.; Membrane protein, multi-pass; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: connexon complex; endoplasmic reticulum membrane; integral to membrane

Molecular Function: protein homodimerization activity

Biological Process: nervous system development; cell-cell signaling; gap junction assembly; transport

Disease: Charcot-marie-tooth Disease, X-linked Dominant, 1