Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Mouse anti-Rat Connective Tissue Growth Factor Monoclonal Antibody | anti-CTGF antibody
Monoclonal Antibody to Connective Tissue Growth Factor (CTGF)
Gene Names
Ctgf; CTGRP
Reactivity
Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
Purity
Protein A + Protein G affinity chromatography
Synonyms
Connective Tissue Growth Factor; Monoclonal Antibody; Monoclonal Antibody to Connective Tissue Growth Factor (CTGF); anti-CTGF antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
A7
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CTGF antibody
Western Blot (WB), Immunohistochemistry (IHC),Immunocytochemistry (ICC),Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-2 ug/mL;1:500-2000
Immunohistochemistry: 5-20 ug/mL;1:50-200
Immunocytochemistry: 5-20 ug/mL;1:50-200
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20 ug/mL;1:50-200
Immunocytochemistry: 5-20 ug/mL;1:50-200
Optimal working dilutions must be determined by end user.
Organism Species
Rattus norvegicus (Rat)
Source
Monoclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant CTGF (Gln25~Ala347) expressed in E.coli
Cross Reactivity
Rat
Conjugate
No Conjugate
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2034997
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Liver Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Liver Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Liver Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Liver Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
connective tissue growth factor
NCBI Official Synonym Full Names
connective tissue growth factor
NCBI Official Symbol
Ctgf
NCBI Official Synonym Symbols
CTGRP
NCBI Protein Information
connective tissue growth factor
UniProt Protein Name
Connective tissue growth factor
Protein Family
UniProt Gene Name
Ctgf
UniProt Synonym Gene Names
Ccn2
NCBI Description
growth factor that may be a mediator of TGFbeta1-driven matrix production and normal skeletal remodeling [RGD, Feb 2006]
Uniprot Description
Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes (). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells (). Enhances fibroblast growth factor-induced DNA synthesis ().
Research Articles on CTGF
Similar Products
Product Notes
The CTGF ctgf (Catalog #AAA2025792) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Connective Tissue Growth Factor (CTGF) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Connective Tissue Growth Factor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC),Immunocytochemistry (ICC),Immunoprecipitation (IP). Western blotting: 0.5-2 ug/mL;1:500-2000 Immunohistochemistry: 5-20 ug/mL;1:50-200 Immunocytochemistry: 5-20 ug/mL;1:50-200 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CTGF ctgf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Connective Tissue Growth Factor, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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