Mouse anti-Human Complement C3a, C3a des-Arg Monoclonal Antibody | anti-C5AR2 antibody

Complement C3a, C3a des-Arg, Human

Cat. Num. AAA602223

• Gene Names: C5AR2; C5L2; GPF77; GPR77
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A/G affinity chromatography from culture supernatant.
• Synonyms: Complement C3a; C3a des-Arg; Monoclonal Antibody; Human; Anti -Complement C3a; anti-C5AR2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 6F68
• Specificity: Recognizes an epitope that is present on human C3, C3a and C3a (desArg). Does not cross-react with C4a or C5a. Recognizes a different epitope on the 9kD C3a than C7850-13J. Effectively inhibits the biological activitiy of C3a in a guinea-pig platelet activation assay.
• Purity/Purification: Affinity Purified; Purified by Protein A/G affinity chromatography from culture supernatant.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide before the addition of glycerol to 40%.

• Applicable Applications for anti-C5AR2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:16,000
• Immunogen: Human C3a.
• Hybridoma: Balb/c mice.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-C5AR2 antibody

Complement C3a is an anaphylatoxin of 77 amino acid residues released by the action of the C3 convertases on the N-terminal of the alpha chain of C3. It is rapidly inactivated by serum carboxypeptidase N which removes the C-terminal arginine residue generating C3a (desArg).

Product Categories/Family for anti-C5AR2 antibody

Antibodies; Abs to Complement

NCBI and Uniprot Product Information

• NCBI GI #: 415712997
• NCBI GeneID: 27202
• NCBI Accession #: NP_001258679.1
• NCBI GenBank Nucleotide #: NM_001271750.1
• UniProt Accession #: Q9P296; B2RA09
• Molecular Weight: 36,080 Da
• NCBI Official Full Name: C5a anaphylatoxin chemotactic receptor 2
• NCBI Official Synonym Full Names: complement component 5a receptor 2
• NCBI Official Symbol: C5AR2
• NCBI Official Synonym Symbols: C5L2; GPF77; GPR77
• NCBI Protein Information: C5a anaphylatoxin chemotactic receptor 2; G protein-coupled receptor 77; G protein-coupled receptor C5L2; C5a anaphylatoxin chemotactic receptor C5L2
• UniProt Protein Name: C5a anaphylatoxin chemotactic receptor 2
• Protein Family: C5a anaphylatoxin chemotactic receptor
• UniProt Gene Name: C5AR2
• UniProt Synonym Gene Names: C5L2; GPR77
• UniProt Entry Name: C5AR2_HUMAN

NCBI Description

This gene encodes a G-protein coupled receptor 1 family member involved in the complement system of the innate immune response. Unlike classical G-protein coupled receptors, the encoded protein does not associate with intracellular G-proteins. It may instead modulate signal transduction through the beta-arrestin pathway, and may alternatively act as a decoy receptor. This gene may be involved in coronary artery disease and in the pathogenesis of sepsis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]

Uniprot Description

C5AR2: Receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides and also for their dearginated forms ASP/C3adesArg, C4adesArg and C5adesArg respectively. Couples weakly to G(i)-mediated signaling pathways. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: apical part of cell; integral to membrane; basal plasma membrane; plasma membrane

Molecular Function: protein binding; C5a anaphylatoxin receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; negative regulation of tumor necrosis factor production; chemotaxis; inflammatory response; positive regulation of epithelial cell proliferation