Rat anti-Human Complement C3, C3c Monoclonal Antibody | anti-C3 antibody

Complement C3, C3c (Complement Component C3c, C3/C3c, Acylation Stimulating Protein Cleavage Product, ASP, Complement Component 3, Complement Factor 3, CPAMD1)

Cat. Num. AAA6009516

• Reactivity: Human
• Applications: Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Complement C3; C3c; Monoclonal Antibody; C3c (Complement Component C3c; C3/C3c; Acylation Stimulating Protein Cleavage Product; ASP; Complement Component 3; Complement Factor 3; CPAMD1); Anti -Complement C3; anti-C3 antibody

• Host: Rat
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 13A36
• Specificity: Recognizes the cleaved human C3 fragment C3c. Recognizes a conformational epitope in C3c, C3b and iC3b.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

• Applicable Applications for anti-C3 antibody: Immunoprecipitation (IP)
• Application Notes: Suitable for use in Immunoprecipitation.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-C3 antibody

The complement system is an important factor in innate immunity. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. Activation products of the complement cascade contain neo-epitopes that are not present in the individual native components. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. C3 is the most abundant protein of the complement system with serum protein levels of about 1.3mg/ml. An inherited deficiency of C3 predisposes the person to frequent bacterial infections. C3 fragments are deposited in tissues at sites of antibody-mediated immunopathology. In ulcerative colitis and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported. Proteolysis by C3-convertases results in the cleavage of C3 into C3a and C3b. C3b becomes attached to immune complexes and is further cleaved into iC3b and C3f. iC3b is further processed into C3c and C3dg.

Product Categories/Family for anti-C3 antibody

Antibodies; Abs to Complement

NCBI and Uniprot Product Information

• NCBI GI #: 99028969
• NCBI GeneID: 280677
• NCBI Accession #: NP_001035559.2
• NCBI GenBank Nucleotide #: NM_001040469.2
• UniProt Accession #: Q2UVX4; Q2KIZ4
• Molecular Weight: 187,253 Da
• NCBI Official Full Name: complement C3 preproprotein
• NCBI Official Synonym Full Names: complement component 3<
• NCBI Official Symbol: C3
• NCBI Protein Information: complement C3
• UniProt Protein Name: Complement C3
• Protein Family: Complement C3
• UniProt Gene Name: C3
• UniProt Synonym Gene Names: ASP
• UniProt Entry Name: CO3_BOVIN

Uniprot Description

Function: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates

By similarity.Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes

By similarity.Acylation stimulating protein: adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-internalization and recycling of C5AR2

By similarity.

Subunit structure: C3 precursor is first processed by the removal of 4 Arg residues, forming two chains, beta and alpha, linked by a disulfide bond. C3 convertase activates C3 by cleaving the alpha chain, releasing C3a anaphylatoxin and generating C3b (beta chain + alpha' chain). C3dg interacts with CR2 (via the N-terminal Sushi domains 1 and 2). Interacts with VSIG4. Interacts (both C3a and ASP) with C5AR2; the interaction occurs with higher affinity for ASP, enhancing the phosphorylation and activation of C5AR2, recruitment of ARRB2 to the cell surface and endocytosis of GRP77

By similarity. Interacts with BHV-1 glycoprotein C. Ref.3

Subcellular location: Secreted.

Post-translational modification: C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g. C3a is further processed by carboxypeptidases to release the C-terminal arginine residue generating the acylation stimulating protein (ASP). Levels of ASP are increased in adipocytes in the postprandial period and by dietary chylomicrons

By similarity.Phosphorylation sites are present in the extracellular medium

By similarity.

Sequence similarities: Contains 1 anaphylatoxin-like domain.Contains 1 NTR domain.