Mouse anti-Human COMMD7 Monoclonal Antibody | anti-COMMD7 antibody

COMMD7 antibody

Cat. Num. AAA200388

• Gene Names: COMMD7; C20orf92; dJ1085F17.3
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: By protein-A affinity chromatography
• Synonyms: COMMD7; Monoclonal Antibody; COMMD7 antibody; COMM domain-containing protein 1; C2orf5; MuRR1; COMMD-1; COMMD 1; COMM; MuRR 1; MuRR-1; anti-COMMD7 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG3, k
• Clone Number: AT49B5
• Purity/Purification: By protein-A affinity chromatography
• Form/Format: Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 200

• Applicable Applications for anti-COMMD7 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Antigen Species: Human
• Immunogen: Recombinant human COMMD7 (1-200aa)purified from E. coli
• Clone Note: Anti-human COMMD7 mAb, clone AT49B5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human COMMD7 protein.
• Preparation and Storage: Can be stored at 4 degree C.; For long term storage, aliquot and store at -20 degree C.; Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human COMMD7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: A549 cell lysate Lane 2.: HepG2 cell lysate)

Immunofluorescence (IF)

(ICC/IF analysis of COMMD7 in A549 cells line, stained with monoclonal anti-human COMMD7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

Related Product Information for anti-COMMD7 antibody

COMMD family members are a group of evolutionary conserved proteins that share a common COMM domain at the extreme C-terminus, which provides an interface for protein-protein interactions. COMMD proteins may also play a role in the function of epithelial sodium channels, cell proliferation, and copper homeostasis and in the regulation of the ubiquitin pathway. With highest expression in lung, COMMD7 (COMM domain-containing protein 7) is a 200 amino acid protein that binds COMMD1 via its COMM domain.

Product Categories/Family for anti-COMMD7 antibody

Signal Transduction

References

Burstein E., et al. (2005) J Biol Chem. 280 (23): 22222-22232.; De Bie P., et al. (2006) Biochem J. 398 (1): 63-71.

NCBI and Uniprot Product Information

• NCBI GI #: 151101369
• NCBI GeneID: 149951
• NCBI Accession #: NP_444269
• NCBI GenBank Nucleotide #: NM_053041.2
• UniProt Accession #: Q86VX2; Q5JYB0; Q96SI7; Q9BW53; A2BHJ2; B3KTZ2
• Molecular Weight: 22,412 Da
• NCBI Official Full Name: COMM domain-containing protein 7 isoform 1
• NCBI Official Synonym Full Names: COMM domain containing 7
• NCBI Official Symbol: COMMD7
• NCBI Official Synonym Symbols: C20orf92; dJ1085F17.3
• NCBI Protein Information: COMM domain-containing protein 7
• UniProt Protein Name: COMM domain-containing protein 7
• Protein Family: COMM domain-containing protein
• UniProt Gene Name: COMMD7
• UniProt Synonym Gene Names: C20orf92

Uniprot Description

May modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes (PubMed:21778237). Associates with the NF-kappa-B complex and suppresses its transcriptional activity (PubMed:15799966).