Mouse Collagen VII Monoclonal Antibody | anti-COL7A1 antibody

Collagen VII

Cat. Num. AAA4380809

• Gene Names: COL7A1; EBD1; EBR1; EBDCT; NDNC8
• Reactivity: Human, Sheep, Goat, Horse, Guinea Pig, Cow
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Collagen VII; Monoclonal Antibody; Alpha 1 type VII collagen; Collagen 7; Collagen alpha 1(VII) chain; Collagen type VII alpha 1; Collagen VII alpha 1 polypeptide Col7a1; EBD1; EBDCT; EBR1; Long-chain (LC) collagen; anti-COL7A1 antibody

• Host: Mouse
• Reactivity: Human, Sheep, Goat, Horse, Guinea Pig, Cow
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: LH7.2
• Specificity: Recognizes an epitope located on collagenase digested Type VII collagen (150kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 2944

• Applicable Applications for anti-COL7A1 antibody: ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
• Application Notes: ELISA: For coating, order antibody without BSA; WB: 0.5-1.0ug/ml; FC/FACS: 0.5-1ug/million cells; IF: 1-2ug/ml; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: HeLa, cells. Human lymphocytes. Human lymph node or tonsil.
• Immunogen: Recombinant full-length human Collagen VII protein
• Cellular Localization: Secreted. Extracellular matrix. Basement Membrane.
• Hu-chromosome Location: 3p21.31
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Skin stained with Collagen VII Mouse Monoclonal Antibody (LH7.2).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Skin stained with Collagen VII Mouse Monoclonal Antibody (LH7.2).)

SDS-Page

(SDS-PAGE Analysis Purified Collagen VII Mouse Monoclonal Antibody (LH7.2). Confirmation of Purity and Integrity of Antibody.)

Immunofluorescence (IF)

(Immunofluorescence Analysis of PFA-fixed HeLa cells labeling Collagen VII using Collagen VII Mouse Monoclonal Antibody (LH7.2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.)

Flow Cytometry (FC/FACS)

(Flow Cytometric Analysis of PFA-fixed HeLa cells using Collagen VII Mouse Monoclonal Antibody (LH7.2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red))

References

Ponsioen TL et al. Mol Vis 14:652-60 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4502961
• NCBI GeneID: 1294
• NCBI Accession #: NP_000085.1
• NCBI GenBank Nucleotide #: NP_000085.1
• UniProt Accession #: Q02388
• Molecular Weight: 290kDa
• NCBI Official Full Name: collagen alpha-1(VII) chain
• NCBI Official Synonym Full Names: collagen type VII alpha 1 chain
• NCBI Official Symbol: COL7A1
• NCBI Official Synonym Symbols: EBD1; EBR1; EBDCT; NDNC8
• NCBI Protein Information: collagen alpha-1(VII) chain
• UniProt Protein Name: Collagen alpha-1(VII) chain
• UniProt Gene Name: COL7A1
• UniProt Synonym Gene Names: LC collagen
• UniProt Entry Name: CO7A1_HUMAN

NCBI Description

This gene encodes the alpha chain of type VII collagen. The type VII collagen fibril, composed of three identical alpha collagen chains, is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in this gene are associated with all forms of dystrophic epidermolysis bullosa. In the absence of mutations, however, an acquired form of this disease can result from an autoimmune response made to type VII collagen. [provided by RefSeq, Jul 2008]

Uniprot Description

COL7A1: the alpha chain of type VII collagen, an extra-cellular basement membrane protein restricted to the zone beneath stratified squamous epithelia. Type VII collagen fibrils are composed of three identical alpha collagen chains. Forms anchoring fibrils, which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen. Dystrophic epidermolysis bullosa can result from defects in this protein or an autoimmune response made to type VII collagen. Homotrimer. Interacts with MIA3, facilitating its loading into transport carriers and subsequent secretion. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Secreted; Secreted, signal peptide; Inhibitor

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: extracellular matrix; extracellular space; collagen type VII; endoplasmic reticulum lumen; extracellular region; basement membrane

Molecular Function: serine-type endopeptidase inhibitor activity; identical protein binding; protein binding

Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; epidermis development; cell adhesion

Disease: Transient Bullous Dermolysis Of The Newborn; Epidermolysis Bullosa Dystrophica, Autosomal Dominant; Nail Disorder, Nonsyndromic Congenital, 8; Epidermolysis Bullosa Dystrophica, Autosomal Recessive; Epidermolysis Bullosa Pruriginosa; Epidermolysis Bullosa Dystrophica, Pretibial; Epidermolysis Bullosa With Congenital Localized Absence Of Skin And Deformity Of Nails