Mouse Collagen IV Monoclonal Antibody | anti-COL4A1 antibody

Collagen IV Mouse Monoclonal Antibody (8E5)

Cat. Num. AAA9700592

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Synonyms: Collagen IV; Monoclonal Antibody; Collagen IV Mouse Monoclonal Antibody (8E5); Mouse Anti-Collagen IV Mouse Monoclonal Antibody (8E5); Collagen alpha-1(IV) chain [Cleaved into: Arresten]; anti-COL4A1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: The antibody detects endogenous Collagen IV protein.
• Purity/Purification: Affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Form/Format: Liquid; PBS, pH 7.4, Containing 0.02% Sodium Azide As Preservative and 50% Glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1669

• Applicable Applications for anti-COL4A1 antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF)
• Application Notes: IHC-P: 1:50-1:200
• Immunogen: Synthetic Peptide of Collagen IV
• Preparation and Storage: Stable for one year at -20 degree C from date of shipment.; For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Immunofluorescence (IF)

(Fig.1. Immunofluorescence analysis of human liver tissue. 1, Collagen IV Mouse Monoclonal Antibody (8E5) (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)

Immunofluorescence (IF)

(Fig.2. Immunofluorescence analysis of mouse spleen tissue. 1, Collagen IV Mouse Monoclonal Antibody (8E5) (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)

Immunofluorescence (IF)

(Fig.3. Immunofluorescence analysis of rat lung tissue. 1, Collagen IV Mouse Monoclonal Antibody (8E5) (red) was diluted at 1:200 (4 degree C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)

Immunohistochemistry (IHC)

(Fig.4. Immunohistochemical analysis of paraffin-embedded human lung tissue. 1, Collagen IV Mouse Monoclonal Antibody (8E5) was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)

Immunohistochemistry (IHC)

(Fig.5. Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, Collagen IV Mouse Monoclonal Antibody (8E5) was diluted at 1:200 (4 degree C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)

Related Product Information for anti-COL4A1 antibody

COL4A1 (collagen type IV alpha 1 chain) encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. COL4A1 shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in COL4A1 cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 148536825
• NCBI Accession #: NP_001836.2
• NCBI GenBank Nucleotide #: NP_001836.2
• UniProt Accession #: P02462
• NCBI Official Full Name: collagen alpha-1(IV) chain preproprotein
• UniProt Protein Name: Collagen alpha-1(IV) chain
• Protein Family: Collagen
• UniProt Gene Name: COL4A1
• UniProt Entry Name: CO4A1_HUMAN

Uniprot Description

COL4A1: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH). Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant. Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC). The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries. Defects in COL4A1 are a cause of familial porencephaly (POREN1). Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles. Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Extracellular matrix

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: extracellular matrix; endoplasmic reticulum lumen; collagen type IV; extracellular region; basement membrane

Molecular Function: protein binding; platelet-derived growth factor binding; extracellular matrix structural constituent; extracellular matrix constituent conferring elasticity

Biological Process: patterning of blood vessels; receptor-mediated endocytosis; extracellular matrix disassembly; axon guidance; collagen catabolic process; extracellular matrix organization and biogenesis; epithelial cell differentiation; blood vessel morphogenesis; brain development; neuromuscular junction development

Disease: Porencephaly 1; Brain Small Vessel Disease With Or Without Ocular Anomalies; Angiopathy, Hereditary, With Nephropathy, Aneurysms, And Muscle Cramps; Hemorrhage, Intracerebral, Susceptibility To; Retinal Arteries, Tortuosity Of