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Western Blot (WB)
(Western Blot detection against immunogen (35.42kD).)
Mouse anti-Human, Mouse COL1A1 Monoclonal Antibody | anti-COL1A1 antibody
COL1A1 (Collagen alpha-1(I) Chain, Alpha-1 Type I Collagen) (HRP)
Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
COL1A1; Monoclonal Antibody; COL1A1 (Collagen alpha-1(I) Chain; Alpha-1 Type I Collagen) (HRP); anti-COL1A1 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG3,k
Clone Number
3G3
Specificity
Recognizes human COL1A1. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Sequence Length
1464
Applicable Applications for anti-COL1A1 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa1021-1108 from human COL1A1 (NP_000079.1) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
EGSPGRDGSPGAKGDRGETGPAGPPGAPGAPGAPGPVGPAGKSGDRGETGPAGPAGPVGPVGARGPAGPQGPRGDKGETGEQGDRGIK
Conjugate
HRP
Preparation and Storage
May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot detection against immunogen (35.42kD).)
Western Blot (WB)
(Western Blot detection against immunogen (35.42kD).)
Western Blot (WB)
(Western Blot analysis of COL1A1 expression in NIH/3T3 using 125176.)
Western Blot (WB)
(Western Blot analysis of COL1A1 expression in NIH/3T3 using 125176.)
Immunohistochemistry (IHC)
(Immunoperoxidase to COL1A1 on formalin-fixed paraffin-embedded human pancreas using 125176 (3ug/ml).)
Immunohistochemistry (IHC)
(Immunoperoxidase to COL1A1 on formalin-fixed paraffin-embedded human pancreas using 125176 (3ug/ml).)
Testing Data
(Detection limit for 125176 is 0.03ng/ml as a capture antibody.)
Testing Data
(Detection limit for 125176 is 0.03ng/ml as a capture antibody.)
Related Product Information for anti-COL1A1 antibody
COL1A1 is the major component of type I collagen, and the sole component of the collagen located in cartilage. COL1A1 mutations are associated with osteogenesis imperfecta type I, II, III, and IV, Ehlers-Danlos syndrome type I and II, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a skin tumor called dermatofibrosarcoma protuberans. Two COL1A1 transcripts arise from variant polyadenylation signals for this gene.
Product Categories/Family for anti-COL1A1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
alpha 1 type I collagen preproprotein
Protein Family
Similar Products
Product Notes
The COL1A1 (Catalog #AAA6151892) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The COL1A1 (Collagen alpha-1(I) Chain, Alpha-1 Type I Collagen) (HRP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's COL1A1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the COL1A1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COL1A1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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