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Western Blot (WB) (Western Blot detection against Immunogen (50.86kD).)

Mouse anti-Human COASY Monoclonal Antibody | anti-COASY antibody

COASY (Bifunctional Coenzyme A Synthase, NBP, POV-2, Phosphopantetheine Adenylyltransferase, Dephospho-CoA Pyrophosphorylase, Pantetheine-phosphate Adenylyltransferase, Dephospho-CoA Kinase, Dephosphocoenzyme A Kinase, FLJ35179) (FITC)

Gene Names
COASY; NBP; DPCK; PPAT; UKR1; NBIA6; pOV-2
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
COASY; Monoclonal Antibody; COASY (Bifunctional Coenzyme A Synthase; NBP; POV-2; Phosphopantetheine Adenylyltransferase; Dephospho-CoA Pyrophosphorylase; Pantetheine-phosphate Adenylyltransferase; Dephospho-CoA Kinase; Dephosphocoenzyme A Kinase; FLJ35179) (FITC); anti-COASY antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1H6
Specificity
Recognizes human COASY.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Applicable Applications for anti-COASY antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-249 from COASY (AAH06354) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MLGNLLRPPYERPELPTCLYVIGLTGISGSGKSSIAQRLKGLGAFVIDSDHLGHRAYAPGGPAYQPVVEAFGTDILHKDGIINRKVLGSRVFGNKKQLKILTDIMWPIIAKLAREEMDRAVAEGKRVCVIDAAVLLEAGWQNLVHEVWTAVIPETEAVRRIVERDGLSEAAAQSRLQSQMSGQQLVEQSHVVLSTLWEPHITQRQVEKAWALLQKRIPKTHQALD
Conjugate
FITC
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (50.86kD).)

Western Blot (WB) (Western Blot detection against Immunogen (50.86kD).)

Western Blot (WB)

(COASY monoclonal antibody, Western Blot analysis of COASY expression in A-431.)

Western Blot (WB) (COASY monoclonal antibody, Western Blot analysis of COASY expression in A-431.)

Western Blot (WB)

(Western Blot analysis of COASY expression in transfected 293T cell line by COASY monoclonal antibody. Lane 1: COASY transfected lysate (30.2kD). Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of COASY expression in transfected 293T cell line by COASY monoclonal antibody. Lane 1: COASY transfected lysate (30.2kD). Lane 2: Non-transfected lysate.)

Testing Data

(Detection limit for recombinant GST tagged COASY is ~0.1ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged COASY is ~0.1ng/ml as a capture antibody.)

Western Blot (WB)

(Western blot analysis of COASY over-expressed 293 cell line, cotransfected with COASY Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with COASY monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Western Blot (WB) (Western blot analysis of COASY over-expressed 293 cell line, cotransfected with COASY Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with COASY monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Related Product Information for anti-COASY antibody
Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. COASY is a bifunctional enzyme that catalyzes the 2 last steps in CoA synthesis. These activities are performed by 2 separate enzymes, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), in prokaryotes (Daugherty et al., 2002 [PubMed 11923312]). [supplied by OMIM].
Product Categories/Family for anti-COASY antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
65,339 Da
NCBI Official Full Name
Homo sapiens Coenzyme A synthase, mRNA
NCBI Official Synonym Full Names
Coenzyme A synthase
NCBI Official Symbol
COASY
NCBI Official Synonym Symbols
NBP; DPCK; PPAT; UKR1; NBIA6; pOV-2
NCBI Protein Information
bifunctional coenzyme A synthase

NCBI Description

Coenzyme A (CoA) functions as a carrier of acetyl and acyl groups in cells and thus plays an important role in numerous synthetic and degradative metabolic pathways in all organisms. In eukaryotes, CoA and its derivatives are also involved in membrane trafficking and signal transduction. This gene encodes the bifunctional protein coenzyme A synthase (CoAsy) which carries out the last two steps in the biosynthesis of CoA from pantothenic acid (vitamin B5). The phosphopantetheine adenylyltransferase domain of this bifunctional protein catalyzes the conversion of 4'-phosphopantetheine into dephospho-coenzyme A (dpCoA) while its dephospho-CoA kinase domain completes the final step by phosphorylating dpCoA to form CoA. Mutations in this gene are associated with neurodegeneration with brain iron accumulation (NBIA). Alternative splicing results in multiple isoforms. [provided by RefSeq, Apr 2014]

Research Articles on COASY

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Product Notes

The COASY (Catalog #AAA6146581) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The COASY (Bifunctional Coenzyme A Synthase, NBP, POV-2, Phosphopantetheine Adenylyltransferase, Dephospho-CoA Pyrophosphorylase, Pantetheine-phosphate Adenylyltransferase, Dephospho-CoA Kinase, Dephosphocoenzyme A Kinase, FLJ35179) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COASY can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the COASY for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COASY, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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