Western Blot (WB)
(Western Blot detection against Immunogen (36.74kD).)
Mouse anti-Human CNOT3 Monoclonal Antibody | anti-CNOT3 antibody
CNOT3 (KIAA0691, LENG2, NOT3, CCR4-NOT Transcription Complex Subunit 3, CCR4-associated Factor 3, Leukocyte Receptor Cluster Member 2)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: Immunofluorescence: 10ug/ml
Western Blot (WB)
(Western Blot detection against Immunogen (36.74kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (36.74kD).)
Western Blot (WB)
(CNOT3 monoclonal antibody Western Blot analysis of CNOT3 expression in HeLa NE..)
Western Blot (WB)
(CNOT3 monoclonal antibody Western Blot analysis of CNOT3 expression in HeLa NE..)
Western Blot (WB)
(Western Blot analysis of CNOT3 expression in transfected 293T cell line by CNOT3 monoclonal antibody.|Lane 1: CNOT3 transfected lysate (82kD).|Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of CNOT3 expression in transfected 293T cell line by CNOT3 monoclonal antibody.|Lane 1: CNOT3 transfected lysate (82kD).|Lane 2: Non-transfected lysate.)
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to CNOT3 on HeLa cell. [antibody concentration 10ug/ml])
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to CNOT3 on HeLa cell. [antibody concentration 10ug/ml])
Testing Data
(Detection limit for recombinant GST tagged CNOT3 is 0.3ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged CNOT3 is 0.3ng/ml as a capture antibody.)
NCBI and Uniprot Product Information
Uniprot Description
Function: Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of emryonic stem (ES) cell identity. Ref.6 Ref.13 Ref.14
Subunit structure: Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. In the complex interacts directly with CNOT2. Interacts with TIP120B and NANOS2. Ref.1 Ref.5 Ref.9 Ref.11
Subcellular location: Cytoplasm
Probable. Nucleus
Probable. Cytoplasm › P-body
By similarity. Note: NANOS2 promotes its localization to P-body
By similarity.
Tissue specificity: Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Ref.1
Developmental stage: Expressed in embryonic stem (ES) cells. Ref.14
Sequence similarities: Belongs to the CNOT2/3/5 family.
Sequence caution: The sequence BAA31666.2 differs from that shown. Reason: Erroneous initiation.
Research Articles on CNOT3
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Product Notes
The CNOT3 cnot3 (Catalog #AAA648321) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CNOT3 (KIAA0691, LENG2, NOT3, CCR4-NOT Transcription Complex Subunit 3, CCR4-associated Factor 3, Leukocyte Receptor Cluster Member 2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CNOT3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF). Suitable for use in Immunofluorescence, ELISA and Western Blot. Dilution: Immunofluorescence: 10ug/ml. Researchers should empirically determine the suitability of the CNOT3 cnot3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MADKRKLQGE IDRCLKKVSE GVEQFEDIWQ KLHNAANANQ KEKYEADLKK EIKKLQRLRD QIKTWVASNE IKDKRQLIDN RKLIETQMER FKVVERETKT. It is sometimes possible for the material contained within the vial of "CNOT3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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