Western Blot (WB)
(Western blot detection of Clusterin alpha chain in Rat Brain lysates using Clusterin alpha chain Rabbit mAb(1:1000 diluted). Predicted band size:52kDa. Observed band size:42kDa.)
Rabbit anti-Mouse, Rat Clusterin alpha Chain Monoclonal Antibody | anti-Clu antibody
Clusterin alpha Chain Rabbit mAb
IHC: 1:2500
IP: 1:20
Western Blot (WB)
(Western blot detection of Clusterin alpha chain in Rat Brain lysates using Clusterin alpha chain Rabbit mAb(1:1000 diluted). Predicted band size:52kDa. Observed band size:42kDa.)
Western Blot (WB)
(Western blot detection of Clusterin alpha chain in Rat Brain lysates using Clusterin alpha chain Rabbit mAb(1:1000 diluted). Predicted band size:52kDa. Observed band size:42kDa.)
Functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Promotes apoptosis when in the nucleus. Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. [provided by RefSeq, May 2011]
Uniprot Description
CLU: Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress- induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. Belongs to the clusterin family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Apoptosis; Secreted; Mitochondrial
Cellular Component: extracellular matrix; extracellular space; neuron projection; growth cone; intracellular membrane-bound organelle; membrane; mitochondrion; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; extracellular region; cytoplasmic vesicle; nucleus
Molecular Function: protein binding; ubiquitin protein ligase binding; protein N-terminus binding; misfolded protein binding
Biological Process: cell death; response to misfolded protein; positive regulation of nitric oxide biosynthetic process; protein stabilization; positive regulation of apoptosis; cell morphogenesis; microglial cell activation; positive regulation of tumor necrosis factor production; endocrine pancreas development; activation of NF-kappaB transcription factor; negative regulation of protein homooligomerization; myelin maintenance in the central nervous system; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein import; positive regulation of cell proliferation; chaperone-mediated protein complex assembly; positive regulation of cell differentiation; neurite morphogenesis; negative regulation of apoptosis
Research Articles on Clu
Similar Products
Product Notes
The Clu clu (Catalog #AAA4752665) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Clusterin alpha Chain Rabbit mAb reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Clusterin alpha Chain can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:1000 IHC: 1:2500 IP: 1:20. Researchers should empirically determine the suitability of the Clu clu for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Clusterin alpha Chain, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
