Rat anti-Mouse CLEC9A Monoclonal Antibody | anti-CLEC9A antibody
CLEC9A
Gene Names
Clec9a; DNGR-1; 9830005G06Rik
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunofluorescence
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
CLEC9A; Monoclonal Antibody; C type lectin domain family 9; member A; C-type lectin domain family 8 member A; DNGR1; HEEE9341; PRO34046; UNQ9341; CD370; anti-CLEC9A antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
1F6
Specificity
This Mab recognizes Mouse DNGR-1, which is also known as CLEC9A, which is a highly specific marker of the CD8a+ and the CD103+ DC subsets, as well as a receptor able to recognize a preformed signal exposed on necrotic cells. In peripheral blood, it is highly restricted on the surface of BDCA31(+) dendritic cells and on a small subset of CD14(+) and CD16(-) monocytes.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
241
Applicable Applications for anti-CLEC9A antibody
Flow Cytometry (FC/FACS), Western Blot (WB), Immunofluorescence (IF)
Application Notes
FC/FACS: 0.5-1ug/million cells
WB: 0.5-1ug/ml
IF: 0.5-1ug/ml
WB: 0.5-1ug/ml
IF: 0.5-1ug/ml
Positive Control
Mouse spleen.
Immunogen
RBL-2H3 cells expressing Mouse CLEC9A fused to an HA epitope.
Cellular Localization
Membrane
Hu-chromosome Location
6,63.30 (Mouse)
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
References
1. Sancho et al. 2009. Nature. 458:899-903. 2. Sancho et al. 2008. J Clin Invest 118:2098-110
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27kDa
NCBI Official Full Name
C-type lectin domain family 9 member A
NCBI Official Synonym Full Names
C-type lectin domain family 9, member a
NCBI Official Symbol
Clec9a
NCBI Official Synonym Symbols
DNGR-1; 9830005G06Rik
NCBI Protein Information
C-type lectin domain family 9 member A
UniProt Protein Name
C-type lectin domain family 9 member A
Protein Family
UniProt Gene Name
CLEC9A
UniProt Entry Name
CLC9A_HUMAN
Uniprot Description
CLEC9A: Functions as an endocytic receptor on a small subset of myeloid cells and induces intracellular signaling via spleen tyrosine kinase (Syk). Acts as a receptor for necrotic cells and can mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.
Protein type: Receptor, misc.; Membrane protein, integral
Chromosomal Location of Human Ortholog: 12p13.2
Cellular Component: cell surface; integral to membrane
Molecular Function: carbohydrate binding
Biological Process: receptor-mediated endocytosis; positive regulation of cytokine secretion
Research Articles on CLEC9A
Similar Products
Product Notes
The CLEC9A clec9a (Catalog #AAA4381456) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The CLEC9A reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CLEC9A can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB), Immunofluorescence (IF). FC/FACS: 0.5-1ug/million cells WB: 0.5-1ug/ml IF: 0.5-1ug/ml. Researchers should empirically determine the suitability of the CLEC9A clec9a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLEC9A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
