Mouse anti-Human CLEC4A Monoclonal Antibody | anti-CLEC4A antibody

Anti-CLEC4A Antibody, Mouse Monoclonal

Cat. Num. AAA8105345

• Gene Names: CLEC4A; DCIR; LLIR; DDB27; CLECSF6; HDCGC13P
• Reactivity: Human
• Applications: ELISA, ELISA
• Purity: Protein A
• Synonyms: CLEC4A; Monoclonal Antibody; Anti-CLEC4A Antibody; Mouse Monoclonal; CLEC4A/CLECSF6/DCIR Antibody; Mouse MAb; C-type lectin domain family 4 member A; Anti-CD367 Antibody; Anti-CLECSF6 Antibody; Anti-DCIR Antibody; Anti-DDB27 Antibody; Anti-HDCGC13P Antibody; Anti-LLIR Antibody; anti-CLEC4A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 7D4B5
• Specificity: Human DCIR/CLEC4A
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CLEC4A antibody: ELISA (EIA), ELISA (EIA) (Det)
• Application Notes: ELISA: 1:1000-1:2000; ELISA (Det): 1:1000-1:10,000; ELISA (Det): This antibody will detect Human DCIR/CLEC4A/CLECSF6 in ELISA pair set.; In a sandwich ELISA, it can be used as detection antibody when paired with.
• Immunogen: Recombinant Human CLEC4A protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CLEC4A (rh CLEC4A). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-CLEC4A antibody

Dendritic cell immunoreceptor (DCIR), also known as C-type lectin domain family 4 member A (CLEC4A), C-type lectin superfamily member 6 (CLECSF6), is a single-pass type II C-type lectin receptor expressed mainly in dendritic cells (DCs), which is a negative regulator of DC expansion and has a crucial role in maintaining the homeostasis of the immune system. The Dectin-2 family of C-type lectins that includes Dectin-2, BDCA-2, DCIR, DCAR, Clecsf8 and Mincle. These type II receptors contain a single extracellular carbohydrate recognition domain and have diverse functions in both immunity and homeostasis. DCIR is the only member of the family which contains a cytoplasmic signalling motif and has been shown to act as an inhibitory receptor, while BDCA-2, Dectin-2, DCAR and Mincle all associate with FcRgamma chain to induce cellular activation, including phagocytosis and cytokine production. Dectin-2 and Mincle have been shown to act as pattern recognition receptors for fungi, while DCIR acts as an attachment factor for HIV. In addition to pathogen recognition, DCIR has been shown to be pivotal in preventing autoimmune disease by controlling dendritic cell proliferation. DCIR expressed on antigen presenting cells and granulocytes and acts as an inhibitory receptor via an intracellular immunoreceptor tyrosine-based inhibitory motif (ITIM). It may also be involved via its ITIM motif in the inhibition of B-cell-receptor-mediated calcium mobilization and protein tyrosine phosphorylation. Additionally, DCIR can participate in the capture of HIV-1 and promote infection in trans and in cis of autologous CD4(+) T cells from human immature monocyte-derived DCs. DCIR acts as a ligand for HIV-1 and is involved in events leading to productive virus infection.

Product Categories/Family for anti-CLEC4A antibody

ITIM/ITAM Immunoreceptors and Related Molecules

References

Kanazawa N, et al. (2004) Signaling and immune regulatory role of the dendritic cell immunoreceptor (DCIR) family lectins: DCIR, DCAR, dectin-2 and BDCA-2. Immunobiology. 209(1-2): 179-90.Fujikado N, et al. (2008) Dcir deficiency causes development of autoimmune diseases in mice due to excess expansion of dendritic cells. Nat Med. 14(2): 176-80.Lambert AA, et al. (2008) The C-type lectin surface receptor DCIR acts as a new attachment factor for HIV-1 in dendritic cells and contributes to trans-and cis-infection pathways. Blood. 112(4): 1299-307.Graham LM, et al. (2009) The Dectin-2 family of C-type lectins in immunity and homeostasis. Cytokine. 48(1-2): 148-55.

NCBI and Uniprot Product Information

• NCBI GI #: 7705338
• NCBI GeneID: 50856
• NCBI Accession #: NP_057268.1
• NCBI GenBank Nucleotide #: NM_016184.3
• UniProt Accession #: Q9UMR7; Q17R69; Q8WXW9; Q9H2Z9; Q9NS33; Q9UI34
• NCBI Official Full Name: C-type lectin domain family 4 member A isoform 1
• NCBI Official Synonym Full Names: C-type lectin domain family 4, member A
• NCBI Official Symbol: CLEC4A
• NCBI Official Synonym Symbols: DCIR; LLIR; DDB27; CLECSF6; HDCGC13P
• NCBI Protein Information: C-type lectin domain family 4 member A; C-type lectin DDB27; lectin-like immunoreceptor; dendritic cell immunoreceptor; C-type lectin superfamily member 6; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 6
• UniProt Protein Name: C-type lectin domain family 4 member A
• Protein Family: C-type lectin domain family
• UniProt Gene Name: CLEC4A
• UniProt Synonym Gene Names: CLECSF6; DCIR; LLIR
• UniProt Entry Name: CLC4A_HUMAN

NCBI Description

This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may play a role in inflammatory and immune response. Multiple transcript variants encoding distinct isoforms have been identified for this gene. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]

Uniprot Description

CLEC4A: May be involved in regulating immune reactivity. May play a role in modulating dendritic cells (DC) differentiation and/or maturation. May be involved via its ITIM motif (immunoreceptor tyrosine-based inhibitory motifs) in the inhibition of B-cell-receptor-mediated calcium mobilization and protein tyrosine phosphorylation. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: integral to plasma membrane

Molecular Function: transmembrane receptor activity; carbohydrate binding

Biological Process: cell surface receptor linked signal transduction; innate immune response; cell adhesion