Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded Human Pancreas stained with Clathrin, LC Monoclonal Antibody (SPM174).)
Mouse Clathrin, Light Chain Monoclonal Antibody | anti-CLTA antibody
Clathrin, Light Chain
Gene Names
CLTA; LCA
Reactivity
Human. Others not known.
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
Synonyms
Clathrin; Light Chain; Monoclonal Antibody; Clathrin light chain A; Clathrin light chain B; Clathrin light chain LCA; Clathrin light chain LCB; Clathrin light polypeptide A; Clathrin light polypeptide B; light polypeptide (Lca); light polypeptide (Lcb); CLTA; CLTB; anti-CLTA antibody
Host
Mouse
Reactivity
Human. Others not known.
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
SPM174
Specificity
Recognizes proteins of 31-44kDa, which are identified as Clathrin Light Chains (both A & B). Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60% homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
236
Applicable Applications for anti-CLTA antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes
FC/FACS: 0.5-1ug/million cells
IF: 1-2ug/ml
WB: 0.5-1ug/ml
IHC-F: 0.5-1ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
IF: 1-2ug/ml
WB: 0.5-1ug/ml
IHC-F: 0.5-1ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Immunogen
Purified recombinant N-terminal fragment of human Clathrin Light Chain
Cellular Localization
Cytoplasmic
Hu-chromosome Location
9p13.3
Positive Control
HeLa cells. Placenta or Prostate Carcinoma.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded Human Pancreas stained with Clathrin, LC Monoclonal Antibody (SPM174).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded Human Pancreas stained with Clathrin, LC Monoclonal Antibody (SPM174).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with p27 Monoclonal Antibody (SX53G8))
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with p27 Monoclonal Antibody (SX53G8))
References
Jackson, A.P., et al. 1988. Structure of human clathrin light chains. Conservation of light chain polymorphism in three mammalian species. J. Biol. Chem. 263: 16688-16695.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31-44kDa
NCBI Official Full Name
clathrin light chain A isoform c
NCBI Official Synonym Full Names
clathrin light chain A
NCBI Official Symbol
CLTA
NCBI Official Synonym Symbols
LCA
NCBI Protein Information
clathrin light chain A
UniProt Protein Name
Clathrin light chain A
Protein Family
UniProt Gene Name
CLTA
UniProt Synonym Gene Names
Lca
NCBI Description
Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 8 and 12. [provided by RefSeq, May 2010]
Uniprot Description
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (PubMed:15858577, PubMed:21297582).
Research Articles on CLTA
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Product Notes
The CLTA clta (Catalog #AAA439879) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Clathrin, Light Chain reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's Clathrin, Light Chain can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin. FC/FACS: 0.5-1ug/million cells IF: 1-2ug/ml WB: 0.5-1ug/ml IHC-F: 0.5-1ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Researchers should empirically determine the suitability of the CLTA clta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Clathrin, Light Chain, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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