ELISA (EIA)
(Detection limit of 3ng/ml using MBS605352 as a capture antibody in Sandwich ELISA.)
Mouse CKTSF1B1 Monoclonal Antibody
CKTSF1B1 (Gremlin1, DRM, Cysteine knot superfamily 1 BMP antagonist 1, Increased in high glucose protein 2, IHG-2, Down-regulated in Mos-transformed cells protein, DAN domain family member 2, Cell proliferation-inducing gene 2 protein GREM1, CKTSF1B1, DAN
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
CKTSF1B1; Monoclonal Antibody; CKTSF1B1 (Gremlin1; DRM; Cysteine knot superfamily 1 BMP antagonist 1; Increased in high glucose protein 2; IHG-2; Down-regulated in Mos-transformed cells protein; DAN domain family member 2; Cell proliferation-inducing gene 2 protein GREM1; DAN; Anti -CKTSF1B1 (Gremlin1; DA; anti-CKTSF1B1 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Clone Number
9G339
Specificity
Recognizes human Gremlin 1. Species Crossreactivity: mouse and rat.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.
Applicable Applications for anti-CKTSF1B1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: Direct or Sandwich
Dilution: ELISA: Direct or Sandwich
Immunogen
Partial recombinant corresponding to aa75-185 of human Gremlin-1 (GST). Accession Number: GREM1_HUMAN
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
ELISA (EIA)
(Detection limit of 3ng/ml using MBS605352 as a capture antibody in Sandwich ELISA.)
ELISA (EIA)
(Detection limit of 3ng/ml using MBS605352 as a capture antibody in Sandwich ELISA.)
Western Blot (WB)
(Western Blot detection of GST tagged recombinant human Gremlin-1 using MBS605352. Note: the GST tag alone is 26kD.)
Western Blot (WB)
(Western Blot detection of GST tagged recombinant human Gremlin-1 using MBS605352. Note: the GST tag alone is 26kD.)
Related Product Information for anti-CKTSF1B1 antibody
Gremlin-1 is a secreted cytokine that may play a role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. It down-regulates the BMP4 signaling in a dose-dependent manner. It also acts as inhibitor of monocyte chemotaxis. It is highly expressed in small intestine, fetal brain and colon. It is weakly expressed in brain, ovary, prostate, pancreas and skeletal muscle. In brain found in the region localized around the internal capsule in the large subcortical nuclei, including caudate, putamen, substantia nigra, thalamus and subthalamus. Predominantly expressed in normal cells including neurons, astrocytes and fibroblasts. Gremlin-1 is induced through TGF-beta 1-mediated pathways in Mesangial cell and it is down-regulated in tumor cell lines.
Product Categories/Family for anti-CKTSF1B1 antibody
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Product Notes
The CKTSF1B1 (Catalog #AAA605352) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CKTSF1B1 (Gremlin1, DRM, Cysteine knot superfamily 1 BMP antagonist 1, Increased in high glucose protein 2, IHG-2, Down-regulated in Mos-transformed cells protein, DAN domain family member 2, Cell proliferation-inducing gene 2 protein GREM1, CKTSF1B1, DAN reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CKTSF1B1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: Direct or Sandwich. Researchers should empirically determine the suitability of the CKTSF1B1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CKTSF1B1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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