Western Blot (WB)
(Western Blot detection against Immunogen (67.65kD).)
Mouse anti-Human CK-BB Monoclonal Antibody | anti-CK-BB antibody
CK-BB (CKBB, Creatine Kinase B-type, B-CK, Creatine Kinase B Chain, CKB) (FITC)
Gene Names
CKB; BCK; B-CK; CKBB; HEL-211; HEL-S-29
Reactivity
Human
Applications
ELISA, Immunofluorescence, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CK-BB; Monoclonal Antibody; CK-BB (CKBB; Creatine Kinase B-type; B-CK; Creatine Kinase B Chain; CKB) (FITC); anti-CK-BB antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3D5-3D11
Specificity
Recognizes human CKB.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Applicable Applications for anti-CK-BB antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes
IF: 30ug/ml
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-381 from human CKB (AAH01190) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MPFSNSHNALKLRFPAEDEFPDLSAHNNHMAKVLTPELYAELRAKSTPSGFTLDDVIQTGVDNPGHPYIMTVGCVAGDEESYEVFKDLFDPIIEDRHGGYKPSDEHKTDLNPDNLQGGDDLDPNYVLSSRVRTGRSIRGFCLPPHCSRGERRAIEKLAVEALSSLDGDLAGRYYALKSMTEAEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKTFLVWVNEEDHLRVISMQKGGNMKEVFTRFCTGLTQIETLFKSKDYEFMWNPHLGYILTCPSNLGTGLRAGVHIKLPNLGKHEKFSEVLKRLRLQKRGTGGVDTAAVGGVFDVSNADRLGFSEVELVQMVVDGVKLLIEMEQRLEQGQAIDDLMPAQK
Conjugate
FITC
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (67.65kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (67.65kD).)
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to CKB on HeLa cell. [antibody concentration 30ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to CKB on HeLa cell. [antibody concentration 30ug/ml].)
Product Categories/Family for anti-CK-BB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
42,644 Da
NCBI Official Full Name
Homo sapiens creatine kinase, brain, mRNA
NCBI Official Synonym Full Names
creatine kinase B
NCBI Official Symbol
CKB
NCBI Official Synonym Symbols
BCK; B-CK; CKBB; HEL-211; HEL-S-29
NCBI Protein Information
creatine kinase B-type
NCBI Description
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq, Jul 2008]
Research Articles on CK-BB
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Product Notes
The CK-BB (Catalog #AAA6146520) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CK-BB (CKBB, Creatine Kinase B-type, B-CK, Creatine Kinase B Chain, CKB) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CK-BB can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). IF: 30ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CK-BB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CK-BB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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