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Immunohistochemistry (IHC) (Anti-CISD1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Mouse anti-Human CISD1 Monoclonal Antibody | anti-CISD1 antibody

Mouse Monoclonal [clone AT1A8] (IgG1,k) to Human CISD1

Gene Names
CISD1; ZCD1; MDS029; C10orf70; mitoNEET
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Protein A Purified
Synonyms
CISD1; Monoclonal Antibody; Mouse Monoclonal [clone AT1A8] (IgG1; k) to Human CISD1; Anti-CISD1 Antibody (clone AT1A8) IHC-plus; CDGSH iron sulfur domain 1; C10orf70; MDS029; ZCD1; MitoNEET; Human CISD1; anti-CISD1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
AT1A8
Specificity
Human CISD1
Purity/Purification
Protein A Purified
Form/Format
Supplied in PBS, pH 7.4, 10% glycerol, 0.02% sodium azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
108
Applicable Applications for anti-CISD1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA, IHC-P (10 ug/ml), WB (1:1000)
Target Species
Human
Immunogen Description
Recombinant human CISD1 (32-108aa) purified from E Coli.
Immunogen Type
Recombinant protein
Immunogen
CISD1 antibody was raised against recombinant human CISD1 (32-108aa) purified from E Coli.
Preparation and Storage
Can be stored at 4 degree C. For long term storage, aliquot and store at -20 degree C. Avoid repeated freezing and thawing cycles.

Immunohistochemistry (IHC)

(Anti-CISD1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC) (Anti-CISD1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Western Blot (WB)

(Western Blot: The extract of Mouse liver (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CISD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody.)

Western Blot (WB) (Western Blot: The extract of Mouse liver (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CISD1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,199 Da
NCBI Official Full Name
CDGSH iron-sulfur domain-containing protein 1
NCBI Official Synonym Full Names
CDGSH iron sulfur domain 1
NCBI Official Symbol
CISD1
NCBI Official Synonym Symbols
ZCD1; MDS029; C10orf70; mitoNEET
NCBI Protein Information
CDGSH iron-sulfur domain-containing protein 1; zinc finger CDGSH-type domain 1; zinc finger, CDGSH-type domain 1; CDGSH iron sulfur domain-containing protein 1
UniProt Protein Name
CDGSH iron-sulfur domain-containing protein 1
UniProt Gene Name
CISD1
UniProt Synonym Gene Names
C10orf70; ZCD1
UniProt Entry Name
CISD1_HUMAN

NCBI Description

This gene encodes a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active [2Fe-2S] cluster. The encoded protein has been localized to the outer membrane of mitochondria and is thought to play a role in regulation of oxidation. Genes encoding similar proteins are located on chromosomes 4 and 17, and a pseudogene of this gene is located on chromosome 2. [provided by RefSeq, Feb 2012]

Uniprot Description

ZCD1: Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation. May be involved in Fe-S cluster shuttling and/or in redox reactions. Belongs to the CISD protein family.

Protein type: Membrane protein, integral; Mitochondrial

Chromosomal Location of Human Ortholog: 10q21.1

Cellular Component: mitochondrial outer membrane; mitochondrion; integral to membrane

Molecular Function: 2 iron, 2 sulfur cluster binding; metal ion binding

Biological Process: regulation of cellular respiration

Research Articles on CISD1

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Product Notes

The CISD1 cisd1 (Catalog #AAA249197) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone AT1A8] (IgG1,k) to Human CISD1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CISD1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA, IHC-P (10 ug/ml), WB (1:1000). Researchers should empirically determine the suitability of the CISD1 cisd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CISD1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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