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ELISA (Control Antigen (100 ng);Purple line: Antigen (10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng).)

Mouse anti-Human CHRNB4 Monoclonal Antibody | anti-CHRNB4 antibody

Mouse Monoclonal Antibody to CHRNB4

Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry, Western Blot
Synonyms
CHRNB4; Monoclonal Antibody; Mouse Monoclonal Antibody to CHRNB4; anti-CHRNB4 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1H11F2
Form/Format
Purified antibody in PBS with 0.05% sodium azide.
Sequence Length
198
Applicable Applications for anti-CHRNB4 antibody
ELISA (EIA); Immunocytochemistry (ICC); Flow Cytometry (FC); Western Blot (WB)
Application Notes
ELISA: 1/10000
ICC: 1/100 - 1/500
FC: 1/200 - 1/400
WB: 1/500 - 1/2000.
Immunogen
Purified recombinant fragment of human CHRNB4 (AA: extra 22-236) expressed in E. Coli.
Preparation and Storage
Shipping Information: This product will ship in a box containing blue ice at a temperature of 4 degree C.

ELISA

(Control Antigen (100 ng);Purple line: Antigen (10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng).)

ELISA (Control Antigen (100 ng);Purple line: Antigen (10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng).)

Western Blot (WB)

(analysis using CHRNB4 mAb against human CHRNB4 (AA: extra 22-236) recombinant protein (Expected MW is 51 kDa).)

Western Blot (WB) (analysis using CHRNB4 mAb against human CHRNB4 (AA: extra 22-236) recombinant protein (Expected MW is 51 kDa).)

Flow cytometric (FC)

(analysis of SK-N-SH cells using CHRNB4 mouse mAb (green) and negative control (red).)

Flow cytometric (FC) (analysis of SK-N-SH cells using CHRNB4 mouse mAb (green) and negative control (red).)

Immunofluorescence (IF)

(analysis of Hela cells using CHRNB4 mouse mAb (green).Blue: DRAQ5 fluorescent DNA dye.Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)

Immunofluorescence (IF) (analysis of Hela cells using CHRNB4 mouse mAb (green).Blue: DRAQ5 fluorescent DNA dye.Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Related Product Information for anti-CHRNB4 antibody
CHRNB4 (Cholinergic Receptor Nicotinic Beta 4 Subunit) is a Protein Coding gene. Diseases associated with CHRNB4 include Substance Dependence and Nicotine Dependence, Protection Against. Among its related pathways are Transmission across Chemical Synapses and Immune System. GO annotations related to this gene include extracellular ligand-gated ion channel activity and ligand-gated ion channel activity. An important paralog of this gene is CHRNB2.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
56.4kDa
NCBI Official Full Name
CHRNB4 protein, partial
NCBI Official Synonym Full Names
cholinergic receptor nicotinic beta 4 subunit
NCBI Official Symbol
CHRNB4
NCBI Protein Information
neuronal acetylcholine receptor subunit beta-4

NCBI Description

This gene is found within a conserved gene cluster and encodes one of the beta subunits of the nicotinic acetylcholine receptor (nAChRs) superfamily which form ligand-gated ion channels with a central pore that forms a cation channel. Neuronal nAChRs are pentameric structures that can be either homomeric or heteromeric, with heteromeric structures containing both alpha and beta subunits. Each subunit contains an extracellular amino terminus and four transmembrane domains. Nicotine is one of the agonists that binds to the receptor. Variants in this gene have been associated with nicotine dependence and lung cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2017]

Research Articles on CHRNB4

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Product Notes

The CHRNB4 (Catalog #AAA8537086) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal Antibody to CHRNB4 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHRNB4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA); Immunocytochemistry (ICC); Flow Cytometry (FC); Western Blot (WB). ELISA: 1/10000 ICC: 1/100 - 1/500 FC: 1/200 - 1/400 WB: 1/500 - 1/2000. Researchers should empirically determine the suitability of the CHRNB4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHRNB4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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