Mouse Chondroitin Sulfate Proteoglycan, Brain Core Protein Monoclonal Antibody | anti-ACAN antibody

Chondroitin Sulfate Proteoglycan, Brain Core Protein (CSPG)

Cat. Num. AAA601083

• Gene Names: ACAN; AGC1; ACAN
• Reactivity: Feline, Ferret, Guinea Pig, Hamster, Human, Macaque, Monkey, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
• Purity: Purified; Purified
• Synonyms: Chondroitin Sulfate Proteoglycan; Brain Core Protein; Monoclonal Antibody; Brain Core Protein (CSPG); Anti -Chondroitin Sulfate Proteoglycan; anti-ACAN antibody

• Host: Mouse
• Reactivity: Feline, Ferret, Guinea Pig, Hamster, Human, Macaque, Monkey, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2Q40
• Specificity: RecognizesChondroitin sulfate proteoglycan (CSPG), brain core protein. Species Crossreactivity: human, rat, squirrel, monkey, macaques, guinea pig, gerbil, hamster, ferret, and feline.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

• Applicable Applications for anti-ACAN antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation.; Dilution: Immunohistochemistry: 1:500-1:2,000 (4% paraformaldehyde fixed frozen tissue).
• Immunogen: Feline spinal cord fixed gray matter.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-ACAN antibody

The NG2 chondroitin sulfate proteoglycan is a valuable marker for many types of immature progenitor cells including pericytes/smooth muscle cells, chondroblasts and oligodendrocyte progenitors. NG2 is often expressed in melanomas, chondrosarcomas, glioblastomas and myeloid leukemias. Studies indicate that NG2 is involved in modulating cellular response to growth factors 3-5 and cell motility; these are critical functions in proliferation and migration of immature progenitor cells and tumor cells. Oligodendrocyte precursor cells (OPCs) are a population of NG2 expressing cells in the brain that accumulate in response to CNS injuries. OPCs, together with microglia and astrocytes, form the glial scar, which is believed to inhibit or prevent axonal regeneration partly by producing growth inhibiting NG2 at the injury site8. It has been suggested that inhibiting NG2 at sites of spinal cord injury may increase beneficial axonal regeneration. Studies suggest that NG2 stimulates tumor growth and neovascularization in glioblastoma multiforme by inhibiting angiostatin and thus promoting angiogenesis. As NG2 is associated with increased tunmor cell proliferation, it may be a potential therapeutic marker in brain tumors.

Product Categories/Family for anti-ACAN antibody

Antibodies; Abs to Carbohydrates; Glycoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 1699007
• NCBI GeneID: 395798
• UniProt Accession #: P07898; Q90810; Q90820; Q90991; Q91047; P79787
• Molecular Weight: 223,493 Da
• NCBI Official Full Name: chondroitin sulfate proteoglycan core protein
• NCBI Official Synonym Full Names: aggrecan
• NCBI Official Symbol: ACAN
• NCBI Official Synonym Symbols: AGC1; ACAN
• NCBI Protein Information: aggrecan core protein; CSPCP; aggrecan 1; cartilage-specific proteoglycan core protein; chondroitin sulfate proteoglycan core protein
• UniProt Protein Name: Aggrecan core protein
• Protein Family: Aggrecan core protein
• UniProt Gene Name: ACAN
• UniProt Synonym Gene Names: AGC1
• UniProt Entry Name: PGCA_CHICK

Uniprot Description

Function: This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.

Subcellular location: Secreted › extracellular space › extracellular matrix

Domain: Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3.

Post-translational modification: Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides.

Involvement in disease: Note=Defects in ACAN are the cause of nanomelia, a lethal connective tissue disorder affecting cartilage development (chondrodystrophy) characterized by shortened and malformed limbs. Aggrecan is truncated at its C-terminus in the CS-2 binding domain and is not anymore secreted from the chondrocytes.

Sequence similarities: Belongs to the aggrecan/versican proteoglycan family.Contains 1 C-type lectin domain.Contains 1 EGF-like domain.Contains 1 Ig-like V-type (immunoglobulin-like) domain.Contains 4 Link domains.Contains 1 Sushi (CCP/SCR) domain.