Mouse anti-Human CHFR Monoclonal Antibody | anti-CHFR antibody

CHFR (E3 Ubiquitin-protein Ligase CHFR, Checkpoint with Forkhead and RING Finger Domains Protein, RING Finger Protein 196, RNF196, FLJ10796, FLJ33629) (PE)

Cat. Num. AAA6157090

• Gene Names: CHFR; RNF116; RNF196
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CHFR; Monoclonal Antibody; CHFR (E3 Ubiquitin-protein Ligase CHFR; Checkpoint with Forkhead and RING Finger Domains Protein; RING Finger Protein 196; RNF196; FLJ10796; FLJ33629) (PE); anti-CHFR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1H3-A12
• Specificity: Recognizes human CHFR.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 3189

• Applicable Applications for anti-CHFR antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 2ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-653 from human CHFR (AAH12072) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFDTSGAGAGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGRERSSSCGSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQEDLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRVEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (97.83kD).)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 2ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged CHFR is ~0.1ng/ml as a capture antibody.)

Western Blot (WB)

(Western blot analysis of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Product Categories/Family for anti-CHFR antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 15082329
• NCBI GeneID: 55743
• NCBI Official Full Name: Homo sapiens checkpoint with forkhead and ring finger domains, mRNA
• NCBI Official Synonym Full Names: checkpoint with forkhead and ring finger domains
• NCBI Official Symbol: CHFR
• NCBI Official Synonym Symbols: RNF116; RNF196
• NCBI Protein Information: E3 ubiquitin-protein ligase CHFR
• Protein Family: E3 ubiquitin-protein ligase

NCBI Description

This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014]