Mouse CFTR Monoclonal Antibody | anti-CFTR antibody

MOUSE ANTI HUMAN CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR

Cat. Num. AAA213380

• Gene Names: CFTR; CF; MRP7; ABC35; ABCC7; CFTR/MRP; TNR-CFTR; dJ760C5.1
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Synonyms: CFTR; Monoclonal Antibody; MOUSE ANTI HUMAN CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; anti-CFTR antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M3A7
• Specificity: This item recognises the human cystic fibrosis transmembrane conductance regulator (CFTR). It recognises an epitope localising to the ABC transporter 2 domain, specifically to a region between amino acids 1370 to 1380.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 1480

• Applicable Applications for anti-CFTR antibody: Immunohistology Frozen*, Immunoprecipitation (IP), Western Blot (WB)*
• Application Notes: Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.; Western Blotting: Minimum Dilution: 10ug/ml; Application Note: This antibody recognises a 170kDa band under native conditions.
• Perservative Stabilisers: 0.1% Sodium Azide (NaN3)
• Immunogen: Fusion protein containing residues 1197-1480 of human CFTR (3).; Buffer Solution
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-CFTR antibody

MBS213380 recognises the human cystic fibrosis transmembrane conductance regulator (CFTR). It recognises an epitope localising to the ABC transporter 2 domain, specifically to a region between amino acids 1370 to 1380.

NCBI and Uniprot Product Information

• NCBI GI #: 90421313
• NCBI GeneID: 1080
• NCBI Accession #: NP_000483.3
• NCBI GenBank Nucleotide #: NM_000492.3
• UniProt Accession #: P13569; Q20BG8; Q20BH2; Q2I0A1; Q2I102
• Molecular Weight: Protein Molecular Weight: 170kDA
• NCBI Official Full Name: cystic fibrosis transmembrane conductance regulator
• NCBI Official Synonym Full Names: cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
• NCBI Official Symbol: CFTR
• NCBI Official Synonym Symbols: CF; MRP7; ABC35; ABCC7; CFTR/MRP; TNR-CFTR; dJ760C5.1
• NCBI Protein Information: cystic fibrosis transmembrane conductance regulator; cAMP-dependent chloride channel; channel conductance-controlling ATPase
• UniProt Protein Name: Cystic fibrosis transmembrane conductance regulator
• Protein Family: Cystic fibrosis transmembrane conductance regulator
• UniProt Gene Name: CFTR
• UniProt Synonym Gene Names: ABCC7; CFTR
• UniProt Entry Name: CFTR_HUMAN

NCBI Description

This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily that is involved in multi-drug resistance. The encoded protein functions as a chloride channel and controls the regulation of other transport pathways. Mutations in this gene are associated with the autosomal recessive disorders cystic fibrosis and congenital bilateral aplasia of the vas deferens. Alternatively spliced transcript variants have been described, many of which result from mutations in this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CFTR: a member of the MRP subfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. Functions as a chloride channel and controls the regulation of other transport pathways. Mutations have been observed in patients with the autosomal recessive disorders cystic fibrosis (CF) and congenital bilateral aplasia of the vas deferens (CBAVD). Alternative splice

Protein type: Channel, chloride; Membrane protein, integral; EC 3.6.3.49; Transporter; Transporter, ABC family; Hydrolase; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q31.2

Cellular Component: recycling endosome; cell surface; protein complex; microvillus; cytoplasmic vesicle membrane; early endosome membrane; basolateral plasma membrane; apical plasma membrane; early endosome; cytoplasm; plasma membrane

Molecular Function: bicarbonate transmembrane transporter activity; protein binding; chloride channel activity; chloride channel inhibitor activity; enzyme binding; chloride transmembrane transporter activity; ATP-binding and phosphorylation-dependent chloride channel activity; channel-conductance-controlling ATPase activity; ATP binding; PDZ domain binding

Biological Process: response to drug; intracellular pH elevation; response to peptide hormone stimulus; cholesterol transport; iodide transport; water transport; transepithelial chloride transport; membrane hyperpolarization; respiratory gaseous exchange; positive regulation of vasodilation; cholesterol biosynthetic process; vasodilation; cellular response to hormone stimulus; response to estrogen stimulus; bicarbonate transport; transport; response to cytokine stimulus; transmembrane transport; sperm capacitation; lung development

Disease: Vas Deferens, Congenital Bilateral Aplasia Of; Bronchiectasis With Or Without Elevated Sweat Chloride 1; Cystic Fibrosis; Pancreatitis, Hereditary