Mouse anti-Human CENPF/CENP-F Monoclonal Antibody | anti-CENPF/CENP-F antibody

Anti-CENPF/CENP-F Antibody (aa1759-2093)

Cat. Num. AAA2400536

• Gene Names: CENPF; CENF; hcp-1; CILD31; STROMS; PRO1779
• Reactivity: Human
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Protein G purified
• Synonyms: CENPF/CENP-F; Monoclonal Antibody; Anti-CENPF/CENP-F Antibody (aa1759-2093); Rabbit Polyclonal; CENPF; AH antigen; Centromere protein F; CENF; CENP-F; Kinetochore protein CENPF; Mitosin; CENP-F kinetochore protein; Hcp-1; PRO1779; anti-CENPF/CENP-F antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, k
• Specificity: Recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation.
• Purity/Purification: Protein G purified
• Form/Format: PBS, pH 7.4
• Sequence Length: 3114

• Applicable Applications for anti-CENPF/CENP-F antibody: Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IHC-Fr: 10 ug/ml; IHC-P: 10 ug/ml; IP: 1-10 ug/ml; WB: 1-10 ug/ml
• Immunogen: GST fusion protein expressed in E. coli corresponding to aa 1759-2093 of human mitosin.
• Conjugation: Unconjugated
• Epitope: aa1759-2093
• Preparation and Storage: Store at -70 degree C, avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-CENPF antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (WB)

(Anti-CENPF antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Related Product Information for anti-CENPF/CENP-F antibody

Required for kinetochore function and chromosome segregation inmitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a linkbetween recycling vesicles and the microtubule network though Its association with STX4 and SNAP25. Acts as a potential inhibitor ofpocket protein -mediated cellular processes during development byregulating the activity of RB proteins during cell division and proliferation.

NCBI and Uniprot Product Information

• NCBI GI #: 55770834
• NCBI GeneID: 1063
• NCBI Accession #: NP_057427.3
• NCBI GenBank Nucleotide #: NM_016343.3
• UniProt Accession #: P49454; Q13171; Q13246; Q5VVM7
• Molecular Weight: 367,764 Da
• NCBI Official Full Name: centromere protein F
• NCBI Official Synonym Full Names: centromere protein F
• NCBI Official Symbol: CENPF
• NCBI Official Synonym Symbols: CENF; hcp-1; CILD31; STROMS; PRO1779
• NCBI Protein Information: centromere protein F
• UniProt Protein Name: Centromere protein F
• UniProt Gene Name: CENPF
• UniProt Synonym Gene Names: CENP-F

NCBI Description

This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq, Jul 2008]

Uniprot Description

Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia.