Mouse cdk8 Monoclonal Antibody | anti-CDK8 antibody

cdk8 (Cyclin-dependent Kinase 8)

Cat. Num. AAA603554

• Gene Names: CDK8; K35; MGC126074; MGC126075
• Applications: ELISA
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: cdk8; Monoclonal Antibody; cdk8 (Cyclin-dependent Kinase 8); Anti -cdk8 (Cyclin-dependent Kinase 8); anti-CDK8 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5i91
• Specificity: Recognizes cdk8 (Cyclin-dependent Kinase 8).
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.

• Applicable Applications for anti-CDK8 antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA.
• Immunogen: Recombinant human CDK8.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CDK8 antibody

CDK8 phosphorylates the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II. It is bound to and is activated by Cyclin C. CDK8 is involved in the control of cell cycle by transcriptional regulation.

Product Categories/Family for anti-CDK8 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 4502745
• NCBI GeneID: 1024
• NCBI Accession #: NP_001251.1
• NCBI GenBank Nucleotide #: NM_001260.1
• UniProt Accession #: P49336; Q5VUF3; Q6ISB5; Q64FY6
• Molecular Weight: 53,284 Da
• NCBI Official Full Name: cyclin-dependent kinase 8
• NCBI Official Synonym Full Names: cyclin-dependent kinase 8
• NCBI Official Symbol: CDK8
• NCBI Official Synonym Symbols: K35; MGC126074; MGC126075
• NCBI Protein Information: cyclin-dependent kinase 8; OTTHUMP00000018158; protein kinase K35; CDK8 protein kinase; mediator complex subunit CDK8; cell division protein kinase 8; mediator of RNA polymerase II transcription subunit CDK8
• UniProt Protein Name: Cyclin-dependent kinase 8
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK8
• UniProt Entry Name: CDK8_HUMAN

NCBI Description

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase and its regulatory subunit cyclin C are components of the RNA polymerase II holoenzyme complex, which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. This kinase has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery. [provided by RefSeq]

Uniprot Description

CDK8: a protein kinase of the CDK family. Along with cyclin C forms part of the RNA polymerase II holoenzyme complex. Phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. Regulates transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery.

Protein type: EC 2.7.11.23; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; Protein kinase, CMGC; Kinase, protein; CMGC group; CDK family; CDK8 subfamily

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: nucleoplasm; Srb-mediator complex; nucleus

Molecular Function: RNA polymerase subunit kinase activity; protein binding; cyclin-dependent protein kinase activity; ATP binding; protein kinase activity

Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; transcription, DNA-dependent; regulation of cell cycle; transforming growth factor beta receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; gene expression; protein amino acid phosphorylation