Rat anti-Mouse CD90/Thy-1 Monoclonal Antibody | anti-THY1 antibody
Rat Anti-Mouse CD90/Thy-1-FITC
Gene Names
THY1; CD90
Reactivity
Mouse
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry
Synonyms
CD90/Thy-1; Monoclonal Antibody; Rat Anti-Mouse CD90/Thy-1-FITC; Rat Anti-Mouse CD90/Thy-1 - FITC; Rat Anti-Mouse CD90/Thy-1; anti-THY1 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2c kappa
Clone Number
G7
Specificity
Thy-1.1 and Thy-1.2 alloantigens (Thy-1 epitope region A)
CD90/Thy-1, a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily of cell surface receptors, consists of a single V-set domain.^1-4 It is expressed on thymocytes, peripheral T lymphocytes, some intraepithelial T lymphocytes and neurons of all mouse strains.^1,2 MAb G7 stimulates T-cell proliferation and IL-2 secretion, via signalling through the T-cell receptor/CD3 complex.^1,5-7 The MAb has also been reported to promote apoptosis of thymocytes and CTL clones,^4,7 and to mediate adhesion of thymocytes to thymic stroma.^8
CD90/Thy-1, a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily of cell surface receptors, consists of a single V-set domain.^1-4 It is expressed on thymocytes, peripheral T lymphocytes, some intraepithelial T lymphocytes and neurons of all mouse strains.^1,2 MAb G7 stimulates T-cell proliferation and IL-2 secretion, via signalling through the T-cell receptor/CD3 complex.^1,5-7 The MAb has also been reported to promote apoptosis of thymocytes and CTL clones,^4,7 and to mediate adhesion of thymocytes to thymic stroma.^8
Form/Format
Fluorescein Conjugate
Sequence Length
161
Applicable Applications for anti-THY1 antibody
Flow Cytometry; Immunocytochemistry; Immunohistochemistry (Acetone Fixed/Frozen Sections)
Application Notes
Research Application(s): In vitro T cell activation
Induction of apoptosis
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Induction of apoptosis
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Gunter, K., T. Malek, and E. Shevach. 1984. T-cell activating properties of an anti-Thy-1 monoclonal antibody. J. Exp. Med. 159:716.
2. Williams, A.F., and J. Gagnon. 1982. Science 216:696.
3. Kroczek, R.A., K.C. Gunter, R.N. Germain, and E.M. Shevach. 1986. Nature 322:181.
4. Hueber, A.O., G. Raposo, M. Pierres, and H.-T. He. 1994. J. Exp. Med. 179:785.
5. Tentori, L., D.M. Pardoll, J.C. Zunigaq, J. Hu-Li, W.E. Paul, J.A. Bluestone, and A.M. Kruisbeek. 1988. J. Immunol. 140:1089.
6. Sugiyama, E., A. Cantagrei, T. Reno, F. Stafford-Brady, E.T.H. Yeh, and J.V. Bonventre. 1990. Cell. Immunol. 130:271.
7. Ucker, D.S., J. Meyers, and P.S. Obermuller. 1992. J. Immunol. 149:1583.
8. He, H.-T., P. Naquet, D. Caillol, and M. Pierres. 1991. J. Exp. Med. 173:515.
9. Kruisbeek, A.M., and E. Shevach. 1991. Proliferative assays for T cell function. In: Current Protocols in Immunology. Coligan, J., A. Kruisbeek, D. Margulies, E.M. Shevach, and W. Strober. John Wiley & Sons, New York, NY, pp. 3.12.1-3.12.14.
2. Williams, A.F., and J. Gagnon. 1982. Science 216:696.
3. Kroczek, R.A., K.C. Gunter, R.N. Germain, and E.M. Shevach. 1986. Nature 322:181.
4. Hueber, A.O., G. Raposo, M. Pierres, and H.-T. He. 1994. J. Exp. Med. 179:785.
5. Tentori, L., D.M. Pardoll, J.C. Zunigaq, J. Hu-Li, W.E. Paul, J.A. Bluestone, and A.M. Kruisbeek. 1988. J. Immunol. 140:1089.
6. Sugiyama, E., A. Cantagrei, T. Reno, F. Stafford-Brady, E.T.H. Yeh, and J.V. Bonventre. 1990. Cell. Immunol. 130:271.
7. Ucker, D.S., J. Meyers, and P.S. Obermuller. 1992. J. Immunol. 149:1583.
8. He, H.-T., P. Naquet, D. Caillol, and M. Pierres. 1991. J. Exp. Med. 173:515.
9. Kruisbeek, A.M., and E. Shevach. 1991. Proliferative assays for T cell function. In: Current Protocols in Immunology. Coligan, J., A. Kruisbeek, D. Margulies, E.M. Shevach, and W. Strober. John Wiley & Sons, New York, NY, pp. 3.12.1-3.12.14.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
17,955 Da
NCBI Official Full Name
CD90 protein
NCBI Official Symbol
THY1
NCBI Official Synonym Symbols
CD90
NCBI Protein Information
thy-1 membrane glycoprotein
UniProt Protein Name
CD90 protein
Protein Family
UniProt Gene Name
CD90
UniProt Entry Name
B9ZSM8_PIG
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Product Notes
The THY1 cd90 (Catalog #AAA673462) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse CD90/Thy-1-FITC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD90/Thy-1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunocytochemistry; Immunohistochemistry (Acetone Fixed/Frozen Sections). Research Application(s): In vitro T cell activation Induction of apoptosis Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells. Researchers should empirically determine the suitability of the THY1 cd90 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD90/Thy-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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