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Immunohistochemistry (IHC) (Anti-CD9 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Mouse CD9 Monoclonal Antibody | anti-CD9 antibody

Mouse Monoclonal [clone HI9a] (IgG1,k) to Human CD9

Gene Names
CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
Reactivity
Bovine, Dog, Horse, Human, Rabbit, Sheep
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity Purified
Synonyms
CD9; Monoclonal Antibody; Mouse Monoclonal [clone HI9a] (IgG1; k) to Human CD9; Anti-CD9 Antibody (clone HI9a) IHC-plus; 5H9 antigen; BA2; BA-2/p24 antigen; CD9 antigen; CD9 antigen (p24); DRAP-27; MIC3; MRP-1; Leukocyte antigen MIC3; Tetraspanin-29; TSPAN29; BTCC-1; CD9 molecule; Motility related protein-1; Motility-related protein; TSPAN-29; Human CD9; anti-CD9 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Bovine, Dog, Horse, Human, Rabbit, Sheep
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
HI9a
Purity/Purification
Affinity Purified
Form/Format
PBS, pH 7.2, 0.09% sodium azide.
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
228
Applicable Applications for anti-CD9 antibody
Immunohistochemistry (IHC - Paraffin), Flow Cytometry (FC/FACS)
Application Notes
Flo (0.5 ug/10E6 cells), IHC-P (10 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Target Species
Human
Preparation and Storage
Store undiluted at 4 degrees C

Immunohistochemistry (IHC)

(Anti-CD9 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC) (Anti-CD9 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Flow Cytometry (FC/FACS)

(Human platelets stained with purified HI9a, then detected with anti-mouse IgGs FITC.)

Flow Cytometry (FC/FACS) (Human platelets stained with purified HI9a, then detected with anti-mouse IgGs FITC.)
Related Product Information for anti-CD9 antibody
CD9 is a member of the tetraspan family of membrane proteins. First described on non-T acute lymphoblastic leukemia cells and developing B-lymphocytes it has since been shown to be involved in platelet activation and aggregation, gamete fusion, cell adhesion, cell motility, tumor metastasis, and intracellular signaling in the nervous system.
Product Categories/Family for anti-CD9 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
928
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,416 Da
NCBI Official Full Name
CD9 antigen
NCBI Official Synonym Full Names
CD9 molecule
NCBI Official Symbol
CD9
NCBI Official Synonym Symbols
MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
NCBI Protein Information
CD9 antigen; 5H9 antigen; tetraspanin-29; BA-2/p24 antigen; CD9 antigen (p24); leukocyte antigen MIC3; motility related protein-1; cell growth-inhibiting gene 2 protein
UniProt Protein Name
CD9 antigen
Protein Family
UniProt Gene Name
CD9
UniProt Synonym Gene Names
MIC3; TSPAN29; MRP-1; Tspan-29
UniProt Entry Name
CD9_HUMAN

NCBI Description

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011]

Uniprot Description

CD9: Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. Belongs to the tetraspanin (TM4SF) family.

Protein type: Membrane protein, multi-pass; Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: extracellular space; platelet alpha granule membrane; focal adhesion; integral to plasma membrane; apical plasma membrane; plasma membrane; vesicle; external side of plasma membrane

Molecular Function: integrin binding; protein binding

Biological Process: platelet activation; fusion of sperm to egg plasma membrane; pathogenesis; multicellular organism reproduction; paranodal junction assembly; negative regulation of cell proliferation; platelet degranulation; single fertilization; brain development; cell motility; cell adhesion; oligodendrocyte development; blood coagulation; response to water deprivation

Research Articles on CD9

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Product Notes

The CD9 cd9 (Catalog #AAA245165) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone HI9a] (IgG1,k) to Human CD9 reacts with Bovine, Dog, Horse, Human, Rabbit, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's CD9 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Flow Cytometry (FC/FACS). Flo (0.5 ug/10E6 cells), IHC-P (10 ug/ml) Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the CD9 cd9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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