Rat CD8b Monoclonal Antibody | anti-CD8b antibody

CD8b, Mouse

Cat. Num. AAA4381530

• Gene Names: Cd8b1; Cd8b; Ly-3; Ly-C; Lyt-3
• Reactivity: Mouse; Does not react with Human.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CD8b; Monoclonal Antibody; Mouse; CD8b antigen; CD8 antigen beta polypeptide 1 (p37); CD8 beta; CD8B1; Leu2; Ly3; LYT3; P37; T lymphocyte surface glycoprotein beta chain; T-cell surface glycoprotein CD8 beta chain; anti-CD8b antibody

• Host: Rat
• Reactivity: Mouse; Does not react with Human.
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: H35-17.2
• Specificity: This monoclonal antibody reacts with the Mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. This monoclonal antibody identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 213

• Applicable Applications for anti-CD8b antibody: Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Functional Studies: Order Antibody without BSA & Azide; FC/FACS: 0.5-1ug/million cells in 0.1ml; IF: 0.5-1ug/ml
• Positive Control: MPBMC's or Thymus.
• Immunogen: Five day mixed leukocyte culture population, C57BL/6 anti-BALB/c
• Cellular Localization: Cell Surface and Secreted
• Hu-chromosome Location: 6 C1; 6 32.14 cM (Mouse)
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified CD8b Rat Monoclonal Antibody (H35-17.2). Confirmation of Integrity and Purity of Antibody.)

References

1. Fujiura Y, Kawaguchi M, Kondo Y, et al. Development of CD8 alpha alpha+ intestinal intraepithelial T cells in beta 2-microglobulin- and/or TAP1-deficient mice. J Immunol. 1996; 156(8):2710-2715. 2. Bierer BE, Sleckman BP, Ratnofsky SE, Burakoff SJ. The biologic roles of CD2, CD4, and CD8 in T-cell activation. Annu Rev Immunol. 1989; 7:579-599

NCBI and Uniprot Product Information

• NCBI GI #: 6753358
• NCBI GeneID: 12526
• NCBI Accession #: NP_033988.1
• NCBI GenBank Nucleotide #: NP_033988.1
• UniProt Accession #: P10300
• Molecular Weight: 37kDa
• NCBI Official Full Name: T-cell surface glycoprotein CD8 beta chain
• NCBI Official Synonym Full Names: CD8 antigen, beta chain 1
• NCBI Official Symbol: Cd8b1
• NCBI Official Synonym Symbols: Cd8b; Ly-3; Ly-C; Lyt-3
• NCBI Protein Information: T-cell surface glycoprotein CD8 beta chain
• UniProt Protein Name: T-cell surface glycoprotein CD8 beta chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: Cd8b
• UniProt Synonym Gene Names: Cd8b1; Ly-3; Lyt-3; Lyt3
• UniProt Entry Name: CD8B_MOUSE

Uniprot Description

CD8B: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding

Biological Process: immune system process