Rat anti-Mouse CD86/B7-2 Monoclonal Antibody | anti-LOC554318 antibody
Rat Anti-Mouse CD86/B7-2-APC
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Synonyms
CD86/B7-2; Monoclonal Antibody; Rat Anti-Mouse CD86/B7-2-APC; Rat Anti-Mouse CD86/B7-2 - Allophycocyanin; Rat Anti-Mouse CD86/B7-2; anti-LOC554318 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat (Louvain) IgG2a kappa
Clone Number
GL1
Specificity
Mouse CD86, the B7-2 co-stimulatory molecule and a ligand for CD28 and CD152/CTLA-4
CD86, also known as B7-2, is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes.^1-5 CD86 expression is rapidly upregulated by B-cell specific stimuli with peak expression at 18-42 hours after stimulation.^1, 5 CD86, along with CD80/B7-1, is an important accessory molecule in T cell costimulation via its interaction with CD28 and CD152/CTLA-4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response.^1-9 The monoclonal antibody GL1 blocks mixed lymphocyte reactions and stimulation of T cells by antigen-presenting cells.^1, 3, 5
CD86, also known as B7-2, is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes.^1-5 CD86 expression is rapidly upregulated by B-cell specific stimuli with peak expression at 18-42 hours after stimulation.^1, 5 CD86, along with CD80/B7-1, is an important accessory molecule in T cell costimulation via its interaction with CD28 and CD152/CTLA-4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response.^1-9 The monoclonal antibody GL1 blocks mixed lymphocyte reactions and stimulation of T cells by antigen-presenting cells.^1, 3, 5
Form/Format
Allophycocyanin (APC) Conjugate
Sequence Length
325
Applicable Applications for anti-LOC554318 antibody
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
Application Notes
Research Application(s): In vivo and in vitro blocking assays
Flow Cytometry Suggested Dilution: <= 0.1 ug/10^6 cells
Flow Cytometry Suggested Dilution: <= 0.1 ug/10^6 cells
Immunogen
LPS-activated mouse B cells
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference. Representative flow data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or aminecontaining buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Hathcock, K., G. Laszlo, C. Pucello, O. Linsley, and R.J. Hodes. 1994. J. Exp. Med. 180:631.
2. Freeman, G.J., F. Borriello, R.J. Hodes, H. Reiser, K.S. Hathcock, G. Laszlo, A.J. McKnight, J. Kim, L. Du, D.B. Lombard, G.S. Gray, L.M. Nadler, and A.H. Sharpe. 1993. Science 262:907.
3. Inaba, K. M. Witmer-Pack, M. Inaba, K.S. Hathcock, H. Sakuta, et al. 1994. J. Exp. Med. 180:1849.
4. Larsen, C.P., S.C. Ritchie, R. Hendrix, P.S. Linsley, K.S. Hathcock, R.J. Hodes, R.P. Lowry, and T.C. Pearson. 1994. J. Immunol. 152:5208.
5. Hathcock, K.S., G. Laszlo, C. Pucillo, P. Linsley, and R.J. Hodes. 1994. J. Exp. Med. 180:631.
6. Laszlo, G., K.S. Hathcock, H.B. Dickler, and R.J. Hodes. 1993. J. Immunol. 150:5252.
7. Hathcock, K., G. Laszlo, H. Dickler, J. Bradshaw, P. Linsley, and R Hodes. 1993. Science 262:905.
8. Thompson, C.B. 1995. Cell 978:982.
9. June, C.H., J.A. Bluestone, L.M. Nadler, and C.B. Thompson. 1994. Immuol. Today 15:321.
10. Han, S. et. al. 1995. J. Immunol. 155:556.
2. Freeman, G.J., F. Borriello, R.J. Hodes, H. Reiser, K.S. Hathcock, G. Laszlo, A.J. McKnight, J. Kim, L. Du, D.B. Lombard, G.S. Gray, L.M. Nadler, and A.H. Sharpe. 1993. Science 262:907.
3. Inaba, K. M. Witmer-Pack, M. Inaba, K.S. Hathcock, H. Sakuta, et al. 1994. J. Exp. Med. 180:1849.
4. Larsen, C.P., S.C. Ritchie, R. Hendrix, P.S. Linsley, K.S. Hathcock, R.J. Hodes, R.P. Lowry, and T.C. Pearson. 1994. J. Immunol. 152:5208.
5. Hathcock, K.S., G. Laszlo, C. Pucillo, P. Linsley, and R.J. Hodes. 1994. J. Exp. Med. 180:631.
6. Laszlo, G., K.S. Hathcock, H.B. Dickler, and R.J. Hodes. 1993. J. Immunol. 150:5252.
7. Hathcock, K., G. Laszlo, H. Dickler, J. Bradshaw, P. Linsley, and R Hodes. 1993. Science 262:905.
8. Thompson, C.B. 1995. Cell 978:982.
9. June, C.H., J.A. Bluestone, L.M. Nadler, and C.B. Thompson. 1994. Immuol. Today 15:321.
10. Han, S. et. al. 1995. J. Immunol. 155:556.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
CD86/B7-2 costimulatory molecule
NCBI Official Symbol
LOC554318
NCBI Protein Information
CD86/B7-2 costimulatory molecule; CD86 antigen; B7-1 costimulatory molecule
Research Articles on LOC554318
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Product Notes
The LOC554318 (Catalog #AAA673456) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse CD86/B7-2-APC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD86/B7-2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation. Research Application(s): In vivo and in vitro blocking assays Flow Cytometry Suggested Dilution: <= 0.1 ug/10^6 cells. Researchers should empirically determine the suitability of the LOC554318 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD86/B7-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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