Mouse anti-Human CD83 Monoclonal Antibody | anti-CD83 antibody

Anti-CD83 Antibody, Mouse Monoclonal

Cat. Num. AAA8105787

• Gene Names: CD83; BL11; HB15
• Reactivity: Human
• Applications: ELISA, ELISA
• Purity: Protein A
• Synonyms: CD83; Monoclonal Antibody; Anti-CD83 Antibody; Mouse Monoclonal; CD83 Antibody; Mouse MAb; CD83 molecule; Anti-BL11 Antibody; Anti-HB15 Antibody; anti-CD83 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: 6H4G10
• Specificity: Human CD83.; No cross-reactivity in ELISA with Mouse CD83, Human cell lysate (293 cell line)
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CD83 antibody: ELISA (EIA), ELISA (EIA) (Det)
• Application Notes: ELISA: 1:1000-1:2000; ELISA (Det): 1:1000-1:10,000; ELISA (Det): This antibody will detect Human CD83 in ELISA pair set.; In a sandwich ELISA, it can be used as detection antibody when paired with.
• Immunogen: Recombinant Human CD83 Protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD83 (rh CD83). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-CD83 antibody

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD83 is considered as a marker of mature dendritic cells as well as an adhesion receptor that binds to resting monocytes and a subset of activated CD8+ T cells. In certain conditions, CD83 tended to dimerize or even multimerize through its aberrant intermolecular disulfide bonds. The injection of CD83-Ig can significantly enhance the rate of tumor growth and inhibit the T cell growth.

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12Scholler N, et al.(2002) Cutting Edge: CD83 Regulates the Development of Cellular Immunity. The Journal of Immunology. 168 (6): 2599-602.Lechmann M, et al.(2002) CD83 on dendritic cells: more than just a marker for maturation. Trends in immunology. 23(6): 273-5.

NCBI and Uniprot Product Information

• NCBI GI #: 94421024
• NCBI GeneID: 9308
• NCBI Accession #: NP_001035370.1
• NCBI GenBank Nucleotide #: NM_001040280.1
• UniProt Accession #: Q01151; Q5THX9
• Molecular Weight: 23,042 Da
• NCBI Official Full Name: CD83 antigen isoform b
• NCBI Official Synonym Full Names: CD83 molecule
• NCBI Official Symbol: CD83
• NCBI Official Synonym Symbols: BL11; HB15
• NCBI Protein Information: CD83 antigen; hCD83; B-cell activation protein; cell surface protein HB15; cell-surface glycoprotein; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily)
• UniProt Protein Name: CD83 antigen
• Protein Family: CD83 antigen
• UniProt Gene Name: CD83
• UniProt Synonym Gene Names: hCD83
• UniProt Entry Name: CD83_HUMAN

NCBI Description

The protein encoded by this gene is a single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The encoded protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

CD83: May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p23

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Biological Process: negative regulation of interleukin-4 production; positive regulation of interleukin-2 production; defense response; positive regulation of CD4-positive, alpha beta T cell differentiation; signal transduction; response to organic cyclic substance; positive regulation of interleukin-10 production; humoral immune response