Mouse anti-Human CD82 Monoclonal Antibody | anti-CD82 antibody

CD82 (CD82 Antigen, C33 Antigen, IA4, Inducible Membrane Protein R2, Metastasis Suppressor Kangai-1, Suppressor of Tumorigenicity 6 Protein, Tetraspanin-27, Tspan-27, KAI1, SAR2, ST6, TSPAN27) (AP)

Cat. Num. AAA6130475

• Gene Names: CD82; R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
• Reactivity: Human
• Applications: ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CD82; Monoclonal Antibody; CD82 (CD82 Antigen; C33 Antigen; IA4; Inducible Membrane Protein R2; Metastasis Suppressor Kangai-1; Suppressor of Tumorigenicity 6 Protein; Tetraspanin-27; Tspan-27; KAI1; SAR2; ST6; TSPAN27) (AP); anti-CD82 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4F2
• Specificity: Recognizes human CD82.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CD82 antibody: ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-267 from CD82 (AAH00726) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MGSACIKVTKYFLFLFNLIFFILGAVILGFGVWILADKSSFISVLQTSSSSLRMGAYVFIGVGAVTMLMGFLGCIGAVNEVRCLLGLYFAFLLLILIAQVTAGALFYFNMGKLKQEMGGIVTELIRDYNSSREDSLQDAWDYVQAQVKCCGWVSFYNWTDNAELMNRPEVTYPCSCEVKGEEDNSLSVRKGFCEAPGNRTQSGNHPEDWPVYQEGCMEKVQAWLQENLGIILGVGVGVAIVELLGMVLSICLCRHVHSEDYSKVPKY
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged CD82 is ~0.3ng/ml as a capture antibody.)

Testing Data

(Proximity Ligation Analysis (PLA) of protein-protein interactions between CD19 and CD82. HeLa cells were stained with CD19 rabbit purified polyclonal 1:1200 and CD82 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Related Product Information for anti-CD82 antibody

CD82 is a 45-90 kD type III tetraspan membrane protein which is encoded by the KAI1 gene. A member of the 4-span transmembrane protein superfamily (TM4SF) CD82 forms a complex with CD37, CD53, CD81, ECM and MHC molecules. CD82 is expressed on monocytes, granulocytes, lymphocytes, epithelial cells, endothelial cells, and fibroblasts and plays a role in signal transduction and adhesion. It has been suggested CD82 functions as a tumor suppressor as loss of expression has been found to promote tumor metastasis.

Product Categories/Family for anti-CD82 antibody

Antibodies; CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 33875790
• NCBI GeneID: 3732
• Molecular Weight: 26,818 Da
• NCBI Official Full Name: Homo sapiens CD82 molecule, mRNA
• NCBI Official Synonym Full Names: CD82 molecule
• NCBI Official Symbol: CD82
• NCBI Official Synonym Symbols: R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
• NCBI Protein Information: CD82 antigen
• Protein Family: CD82 antigen

NCBI Description

This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]