Rat CD80 Monoclonal Antibody | anti-CD80 antibody

RAT ANTI MOUSE CD80:FITC

Cat. Num. AAA214316

• Gene Names: Cd80; B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD80; Monoclonal Antibody; RAT ANTI MOUSE CD80:FITC; anti-CD80 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: RM80
• Form/Format: FITC; Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.1mg/ml (varies by lot)
• Sequence Length: 306

• Applicable Applications for anti-CD80 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.; Flow Cytometry: Maximum Dilution: Neat
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin; Preparation
• Immunogen: BCL1 cells expressing CD80; Fusion Partners
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-CD80 antibody

Rat anti Mouse CD80 antibody, clone RM80 recognizes mouse CD80 (B7-1), a 60kD cell surface glycoprotein which is a member of the CD28/B7 family. In mice, CD80 is expressed on monocytes, peritoneal macrophages and dendritic cells, and expression may be significantly increased upon B lymphocytes by LPS and by IL-4. CD80 has been identified as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4), two structurally similar molecules expressed on T cells. CD28 and CTLA4 are two receptors that have essential but opposing functions in T-cell stimulation. The Interaction of CD80 with CD28 stimulates and sustains T cell responses, whereas the interaction of CD80 with CTLA4 is reported to inhibit T-cell responses.

NCBI and Uniprot Product Information

• NCBI GI #: 112983684
• NCBI GeneID: 12519
• NCBI Accession #: NP_033985.3
• NCBI GenBank Nucleotide #: NM_009855.2
• UniProt Accession #: Q00609; Q61332
• Molecular Weight: 24,051 Da
• NCBI Official Full Name: T-lymphocyte activation antigen CD80
• NCBI Official Synonym Full Names: CD80 antigen
• NCBI Official Symbol: Cd80
• NCBI Official Synonym Symbols: B71; Ly53; TSA1; Cd28l; Ly-53; MIC17
• NCBI Protein Information: T-lymphocyte activation antigen CD80; B7 protein; activation B7-1 antigen
• UniProt Protein Name: T-lymphocyte activation antigen CD80
• Protein Family: T-lymphocyte activation antigen
• UniProt Gene Name: Cd80
• UniProt Synonym Gene Names: B7; B7
• UniProt Entry Name: CD80_MOUSE

Uniprot Description

CD80: Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; T cell costimulation; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell proliferation