Mouse anti-Human CD80 Monoclonal Antibody | anti-CD80 antibody

CD80 Antibody [10A1]

Cat. Num. AAA154578

• Gene Names: CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Protein A purified
• Synonyms: CD80; Monoclonal Antibody; CD80 Antibody [10A1]; CD80 molecule; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1; anti-CD80 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 10A1
• Purity/Purification: Protein A purified
• Form/Format: Liquid; Supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 288

• Applicable Applications for anti-CD80 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: IHC starting at 2-5 ug/mL; IF start at 5 ug/mL; FC/FACS at 10 ug/ml.; ; Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
• Immunogen: CD80 antibody was raised against the extracellular domain of human CD80.
• Conjugate: Unconjugated
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.; As with all antibodies care should be taken to avoid repeated freeze thaw cycles.; Antibodies should not be exposed to prolonged high temperatures.

Immunocytochemistry (IHC)

(Immunocytochemistry of CD80 in transfected HEK293 cells with CD80 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of CD80 in transfected HEK293 cells with CD80 antibody at 2 μg/mL.Red: CD80 Antibody [10A1]Blue: DAPI staining)

Immunofluorescence (IF)

(Immunofluorescence of CD80 in human stomach carcinoma tissue with CD80 antibody at 20 μg/mL.Red: CD80 Antibody [10A1]Blue: DAPI staining)

Immunofluorescence (IF)

(Immunofluorescence of CD80 in human tonsil tissue with CD80 antibody at 2 μg/mL.Red: CD80 Antibody [10A1]Blue: DAPI staining)

Immunohistochemistry (IHC)

(Immunohistochemistry of CD80 in human stomach carcinoma tissue with CD80 antibody at 5 μg/mL. )

Immunohistochemistry (IHC)

(Immunohistochemistry of CD80 in human tonsil tissue with CD80 antibody at 5 μg/mL. )

Flow Cytometry (FC/FACS)

(Flow cytometry analysis of CD80 overexpressing HEK293 cells using CD80 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: CD80 overexpressing HEK293 cells. )

Related Product Information for anti-CD80 antibody

CD80, also known as B7-1, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD86, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).

Product Categories/Family for anti-CD80 antibody

Immunology

References

Lane P. Regulation of T and B cell responses by modulating interactions between CD28/CTLA-4 and their ligands, CD80 and CD86. Ann NY Acad Sci 1997; 815:392-400. Feucht J, Kayser S, Gorodezki D, et al. T-cell responses against CD19+ pediatric acute lymphoblastic leukemia mediated by bispecific T-cell engager (BiTE) are regulated contrarily by PD-L1 and CD80/CD86 on leukemic blasts. Oncotarget 2016; 7:76902-19.

NCBI and Uniprot Product Information

• NCBI GI #: 4885123
• NCBI GeneID: 941
• NCBI Accession #: NP_005182
• NCBI GenBank Nucleotide #: NM_005191.3
• UniProt Accession #: P33681; Q5DTA9; Q5DTB0
• Molecular Weight: Predicted: 32 kDa; Observed: 50 kDa
• NCBI Official Full Name: T-lymphocyte activation antigen CD80
• NCBI Official Synonym Full Names: CD80 molecule
• NCBI Official Symbol: CD80
• NCBI Official Synonym Symbols: B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
• NCBI Protein Information: T-lymphocyte activation antigen CD80
• UniProt Protein Name: T-lymphocyte activation antigen CD80
• Protein Family: T-lymphocyte activation antigen
• UniProt Gene Name: CD80
• UniProt Synonym Gene Names: CD28LG; CD28LG1; LAB7; B7

NCBI Description

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]

Uniprot Description

Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.