Rat anti-Mouse CD8 alpha /Lyt-2 Monoclonal Antibody
Rat Anti-Mouse CD8a/Ly-2-APC/CY7
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Synonyms
CD8 alpha /Lyt-2; Monoclonal Antibody; Rat Anti-Mouse CD8a/Ly-2-APC/CY7; Rat Anti-Mouse CD8a/Ly-2 - Allophycocyanin/Cyanine7-Labeled; Rat Anti-Mouse CD8 alpha /Lyt-2; anti-CD8 alpha /Lyt-2 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat (LOU/Ws1/M) IgG2a kappa
Clone Number
53-6.7
Specificity
alpha chain of the murine CD8 heterodimer, Mr 32-34 kDa
In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an alpha chain (CD8 alpha /Lyt-2) and a beta chain (CD8 beta /Lyt-3); and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the "suppressor/cytotoxic" subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8 alpha without CD8 beta. It has been suggested that CD8^+ beta ^- T cells mature extrathymically, while development of the CD8 alpha ^+ beta ^+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8^+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8 alpha ^+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. 1-8
In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an alpha chain (CD8 alpha /Lyt-2) and a beta chain (CD8 beta /Lyt-3); and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the "suppressor/cytotoxic" subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8 alpha without CD8 beta. It has been suggested that CD8^+ beta ^- T cells mature extrathymically, while development of the CD8 alpha ^+ beta ^+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8^+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8 alpha ^+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. 1-8
Form/Format
Allophycocyanin - Cyanine 7 (APC - CY7) Conjugate
Applicable Applications for anti-CD8 alpha /Lyt-2 antibody
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation
Application Notes
Research Application(s): In vivo and in vitro depletion of CD8 alpha ^+ cells
Fractionation of CD8 alpha ^+ T cells
Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
Fractionation of CD8 alpha ^+ T cells
Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference. Representative data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5), allophycocyanin-Cyanine 5.5 conjugate (APC-CY 5.5), allophycocyanin-Cyanine 7 (APC-CY 7) and R-phycoerythrin-Texas Red (R-PE-TXRD) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5), allophycocyanin-Cyanine 5.5 conjugate (APC-CY 5.5), allophycocyanin-Cyanine 7 (APC-CY 7) and R-phycoerythrin-Texas Red (R-PE-TXRD) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Ledbetter, J. A. and W. E. Seaman. 1982. Immunol. Rev. 68:197.
2. Lefrancois, L. 1991. J. Immunol. 147:1746.
3. Lefrancois, L. 1991. Immunology Today 12:436.
4. Takahasi, K., M. Nakata, T. Tanaka, H. Adachi, H. Nakauchi, H. Yagita, and K. Okumura. 1992. Proc. Natl. Acad. Sci. USA. 89:5557.
5. Mage, M. G. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 3.4.1.
6. Kruisbeek, A. M. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 4.1.1.
7. Swain, S. L., D. P. Dialynas, F. W. Fitch, and M. English. 1984. J. Immunol. 132:1118.
8. Ledbetter, J. A. and L. A. Herzenberg. 1979. Immunol. Rev. 47:63.
9. Ledbetter, J. A., R. V. Rouse, H. S. Micklem, and L. A. Herzenberg. 1980. J. Exp. Med. 152:280.
10. Jia, W. and J. F. Kearney. 1996. Personal communication.
2. Lefrancois, L. 1991. J. Immunol. 147:1746.
3. Lefrancois, L. 1991. Immunology Today 12:436.
4. Takahasi, K., M. Nakata, T. Tanaka, H. Adachi, H. Nakauchi, H. Yagita, and K. Okumura. 1992. Proc. Natl. Acad. Sci. USA. 89:5557.
5. Mage, M. G. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 3.4.1.
6. Kruisbeek, A. M. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 4.1.1.
7. Swain, S. L., D. P. Dialynas, F. W. Fitch, and M. English. 1984. J. Immunol. 132:1118.
8. Ledbetter, J. A. and L. A. Herzenberg. 1979. Immunol. Rev. 47:63.
9. Ledbetter, J. A., R. V. Rouse, H. S. Micklem, and L. A. Herzenberg. 1980. J. Exp. Med. 152:280.
10. Jia, W. and J. F. Kearney. 1996. Personal communication.
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Product Notes
The CD8 alpha /Lyt-2 (Catalog #AAA673198) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse CD8a/Ly-2-APC/CY7 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD8 alpha /Lyt-2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation. Research Application(s): In vivo and in vitro depletion of CD8 alpha ^+ cells Fractionation of CD8 alpha ^+ T cells Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells. Researchers should empirically determine the suitability of the CD8 alpha /Lyt-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD8 alpha /Lyt-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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