Mouse anti-Human CD8 alpha Monoclonal Antibody | anti-CD8A antibody

Anti-CD8 alpha Antibody, Mouse Monoclonal

Cat. Num. AAA8104476

• Gene Names: CD8A; CD8; MAL; p32; Leu2
• Reactivity: Human
• Applications: ELISA
• Purity: Protein A
• Synonyms: CD8 alpha; Monoclonal Antibody; Anti-CD8 alpha Antibody; Mouse Monoclonal; CD8/CD8 alpha/Leu-2 Antibody; Mouse MAb; CD8a molecule; Anti-CD8 Antibody; Anti-Leu-2 Antibody; Anti-Leu2 Antibody; Anti-MAL Antibody; Anti-p32 Antibody; Anti-p32 186910 Antibody; anti-CD8A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 01
• Specificity: Human CD8/CD8 alpha/Leu-2
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CD8A antibody: ELISA (EIA) (Cap)
• Application Notes: ELISA (Cap): This antibody will detect Human CD8/CD8 alpha/Leu-2 in ELISA pair set.; In a sandwich ELISA, it can be used as capture antibody when paired with.
• Immunogen: Recombinant Human CD8/CD8 alpha/Leu-2 Protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD8/CD8 alpha/Leu-2 (rh CD8/CD8 alpha/Leu-2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-CD8A antibody

T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B-and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

References

References Devine, L. et al., 2000, J Immunol. 164 (2): 833-8. Arcaro, A. et al., 2000, J Immunol. 165 (4): 2068-76. Saha, K. et al., 2001, Nat Med. 7 (1): 65-72. Romero, P. et al., 2005, Eur J Immunol. 35 (11): 3092-4.

NCBI and Uniprot Product Information

• NCBI GI #: 22902134
• NCBI GeneID: 925
• NCBI Accession #: NP_001759.3
• NCBI GenBank Nucleotide #: NM_001768.6
• UniProt Accession #: P01732; Q13970; Q4ZG17; B4DT80; D6W5M8
• Molecular Weight: 30,214 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD8 alpha chain isoform 1
• NCBI Official Synonym Full Names: CD8a molecule
• NCBI Official Symbol: CD8A
• NCBI Official Synonym Symbols: CD8; MAL; p32; Leu2
• NCBI Protein Information: T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2
• UniProt Protein Name: T-cell surface glycoprotein CD8 alpha chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD8A
• UniProt Synonym Gene Names: MAL
• UniProt Entry Name: CD8A_HUMAN

NCBI Description

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]