anti-Mouse CD79b (B29, Ig beta) Monoclonal Antibody | anti-CD79B antibody

Hamster Anti-Mouse CD79b/B29-LE/AF

Cat. Num. AAA673613

• Gene Names: CD79B; B29; IGB; AGM6
• Reactivity: Mouse
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
• Synonyms: CD79b (B29; Ig beta); Monoclonal Antibody; Hamster Anti-Mouse CD79b/B29-LE/AF; Hamster Anti-Mouse CD79b/B29 - Low Endotoxin/Azide-Free; Hamster Anti-Mouse CD79b (B29; anti-CD79B antibody

• Host: Armenian Hamster
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Armenian Hamster IgG kappa
• Clone Number: HM79-12
• Specificity: beta chain of the mouse B-cell receptor, Mr 23 kDa; Murine CD79 is a 23/21 kDa disulfide-linked heterodimer composed of an alpha chain (CD79a/mb-1) and a beta chain (CD79b/B29) that associates non-covalently with membrane immunoglobulin (Ig) to form the B cell receptor (BCR) complex. Its expression is restricted to B lymphocytes, first appearing on the surface at the pro-B cell stage prior to productive Ig gene rearrangements and remaining through all stages of B-cell differentiation prior to plasma cells. It has been proposed that the CD79 complex on pro-B cell surfaces may function to induce early B-cell differentiation.1
• Form/Format: Low Endotoxin, Azide - Free (LE/AF)
• Sequence Length: 125

• Applicable Applications for anti-CD79B antibody: Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation; Western Blot
• Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. ; Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

1. Koyama, M., K. Ishihara, H. Karasuyama, J.L. Cordell, A. Iwamoto, and T. Nakamura. 1997. Int. Immunol. 9:1767.
2. Nakamura, T. Personal communication.

NCBI and Uniprot Product Information

• NCBI GI #: 1087009
• NCBI GeneID: 974
• UniProt Accession #: P40259; Q53FS2; Q9BU06; Q6PIS4
• Molecular Weight: 26,048 Da
• NCBI Official Full Name: CD79b
• NCBI Official Synonym Full Names: CD79b molecule, immunoglobulin-associated beta
• NCBI Official Symbol: CD79B
• NCBI Official Synonym Symbols: B29; IGB; AGM6
• NCBI Protein Information: B-cell antigen receptor complex-associated protein beta chain; Ig-beta; B-cell-specific glycoprotein B29; immunoglobulin-associated B29 protein; CD79b antigen (immunoglobulin-associated beta)
• UniProt Protein Name: B-cell antigen receptor complex-associated protein beta chain
• Protein Family: B-cell antigen receptor complex-associated protein
• UniProt Gene Name: CD79B
• UniProt Synonym Gene Names: B29; IGB
• UniProt Entry Name: CD79B_HUMAN

NCBI Description

The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Uniprot Description

Function: Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation. Ref.10 Ref.11 Ref.12

Subunit structure: Heterodimer of alpha and beta chains; disulfide-linked. Part of the B-cell antigen receptor complex where the alpha/beta chain heterodimer is non-covalently associated with an antigen-specific membrane-bound surface immunoglobulin of two heavy chains and two light chains. Ref.14

Subcellular location: Cell membrane; Single-pass type I membrane protein. Note: Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts

Tissue specificity: B-cells.

Post-translational modification: Phosphorylated on tyrosine upon B-cell activation.

Involvement in disease: Defects in CD79B are the cause of agammaglobulinemia type 6 (AGM6) [

MIM:612692]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Ref.15

Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.Contains 1 ITAM domain.