Mouse CD79a Monoclonal Antibody | anti-CD79a antibody

CD79a (B lymphocyte-specific MB1 Protein, B Cell antigen receptor complex-Associated Protein alpha chain, CD79a Molecule immunoglobulin Associated alpha, Ig-alpha, IGA, IgM-alpha, Immunoglobulin-Associated alpha, Ly54, MB-1 Membrane GlycoProtein, Membrane

Cat. Num. AAA6124049

• Gene Names: CD79A; IGA; MB-1
• Reactivity: Bovine, Human, Monkey, Mouse, Porcine, Rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: CD79a; Monoclonal Antibody; CD79a (B lymphocyte-specific MB1 Protein; B Cell antigen receptor complex-Associated Protein alpha chain; CD79a Molecule immunoglobulin Associated alpha; Ig-alpha; IGA; IgM-alpha; Immunoglobulin-Associated alpha; Ly54; MB-1 Membrane GlycoProtein; Membrane; B lymphocyte-specific MB1 Protein; B-cell antigen receptor complex-Associated Protein alpha chain; Membrane-bound immunoglobulin-Associated Protein; Surface IgM-Associated Protein; anti-CD79a antibody

• Host: Mouse
• Reactivity: Bovine, Human, Monkey, Mouse, Porcine, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: SPM550
• Specificity: Recognizes human CD79a. Species Crossreactivity: monkey, porcine, bovine, mouse, rat
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS. No preservative added. BSA-Free. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CD79a antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to aa202-216 (GTYQDVGSLNIADVQ) of human CD79a. Cellular Localization: Cell surface
• Conjugate: APC
• Cellular Localization: Cell Surface
• Positive Control: Daudi or Ramos cells. Germinal center B-cells in a lymph node or tonsil.
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded tonsil (10X) stained with.)

Related Product Information for anti-CD79a antibody

A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. Anti-CD79a is generally used to complement anti-CD20 especially for mature B-cell lymphomas after treatment with Rituximab (anti-CD20). This antibody will stain many of the same lymphomas as anti-CD20, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is anti-CD20. Anti-CD79a also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well.

Product Categories/Family for anti-CD79a antibody

Antibodies; CD Markers

References

1. Mason, DY, et al. 1995. Blood 86: 1453-1459.

NCBI and Uniprot Product Information

• NCBI GI #: 4502685
• NCBI GeneID: 973
• NCBI Accession #: NP_001774.1
• NCBI GenBank Nucleotide #: NM_001783.3
• UniProt Accession #: P11912; P11911
• Molecular Weight: 25,038 Da
• NCBI Official Full Name: B-cell antigen receptor complex-associated protein alpha chain isoform 1
• NCBI Official Synonym Full Names: CD79a molecule, immunoglobulin-associated alpha
• NCBI Official Symbol: CD79A
• NCBI Official Synonym Symbols: IGA; MB-1
• NCBI Protein Information: B-cell antigen receptor complex-associated protein alpha chain; ig-alpha; MB-1 membrane glycoprotein; surface IgM-associated protein; CD79a antigen (immunoglobulin-associated alpha); membrane-bound immunoglobulin-associated protein
• UniProt Protein Name: B-cell antigen receptor complex-associated protein alpha chain
• Protein Family: B-cell antigen receptor complex-associated protein
• UniProt Gene Name: CD79A
• UniProt Synonym Gene Names: IGA; MB1
• UniProt Entry Name: CD79A_HUMAN

NCBI Description

The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-alpha protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Ig-alpha: an integral membrane protein that associates with surface immunoglobulin B lymphocyte antigen receptor multimeric complex. Surface Ig non-covalently associates with 2 other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. Two alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: multivesicular body; integral to membrane; plasma membrane; B cell receptor complex; lipid raft; external side of plasma membrane

Molecular Function: transmembrane receptor activity

Biological Process: B cell proliferation; B cell receptor signaling pathway; B cell activation; B cell differentiation

Disease: Agammaglobulinemia 3, Autosomal Recessive