Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with CD45R antibody and 0.03 ug of Mouse IgG1 kappa Isotype Control (PE) (left) or 0.03 ug of CD66a antibody (PE) (right). Total viable cells were used for analysis.)
Mouse CD66a Monoclonal Antibody | anti-CD66a antibody
CD66a antibody (PE)
Applications
Flow Cytometry, Functional Assay
Synonyms
CD66a; Monoclonal Antibody; CD66a antibody (PE); Monoclonal CD66a; Anti-CD66a; BGP; biliary glycoprotein; CEACAM; CD-66a; CD 66a; anti-CD66a antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CC1
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
200 ug/ml (varies by lot)
Applicable Applications for anti-CD66a antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: 0.25 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Biological Significance
The monoclonal antibody CC1 recognizes CD66a, also known as carcinoembryonic antigen-related cell adhesion molecule 1, biliary glycoprotein, and BPG. Expression of CD66a is found on brush borders, epithelial, and endothelial cells. In hematopoietic cells expression is found abundantly on B cells, as well as some NKs, monocytes, DCs, and granulocytes. Although low levels of mRNA have been identified in T cells in humans, resting mouse T lymphocytes are not reported to express CD66a, as confirmed by lack of staining with CC1 antibody. In humans, expression levels of CD66a have been used to identify malignancies. CD66a plays a key role as a regulator of BCR activation of B lymphocytes. An alternate allele, CEACAM1b, is expressed in SJL mice therefore, CC1 does not stain SJL tissue. The monoclonal CC1 has been shown to block viral infection and also enhance B cell proliferation when combined with IgM crosslinking.
Tag/Conjugate
PE
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with CD45R antibody and 0.03 ug of Mouse IgG1 kappa Isotype Control (PE) (left) or 0.03 ug of CD66a antibody (PE) (right). Total viable cells were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with CD45R antibody and 0.03 ug of Mouse IgG1 kappa Isotype Control (PE) (left) or 0.03 ug of CD66a antibody (PE) (right). Total viable cells were used for analysis.)
Related Product Information for anti-CD66a antibody
Mouse monoclonal CD66a antibody (PE)
Product Categories/Family for anti-CD66a antibody
Similar Products
Product Notes
The CD66a (Catalog #AAA834643) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD66a can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: 0.25 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the CD66a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD66a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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