Mouse CD59 / Complement Regulatory Protein / Protectin Monoclonal Antibody | anti-CD59 antibody

CD59 / Complement Regulatory Protein / Protectin Mouse Monoclonal Antibody

Cat. Num. AAA439044

• Gene Names: CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
• Reactivity: Human.; Does not react with Baboon and Horse.; Others not tested
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: CD59 / Complement Regulatory Protein / Protectin; Monoclonal Antibody; CD59 / Complement Regulatory Protein / Protectin Mouse Monoclonal Antibody; 20kDa homologous restriction factor (HRF20); Complement regulatory protein; Human leukocyte antigen MIC11; MAC-inhibitory protein (MACIP); Membrane attack complex inhibition factor (MACIF); Membrane inhibitor of reactive lysis; MIRL; MSK21; Protectin; T cell activating protein; anti-CD59 antibody

• Host: Mouse
• Reactivity: Human.; Does not react with Baboon and Horse.; Others not tested
• Clonality: Monoclonal
• Isotype: IgM, kappa
• Clone Number: 193-27
• Specificity: Reacts with human CD59, a 20kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein (Workshop VI; Code N-L036). CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb recognizes CD59 transfected cells. It is useful for study on GPI-anchored proteins, PNH and CD59 functions.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 128

• Applicable Applications for anti-CD59 antibody: Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Functional Studies (Order Ab without Azide); Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml)
• Cellular Localization: Cell Surface
• Immunogen: Stimulated human leukocytes
• Hu-Chromosome Location: 11p13
• Positive Control: Daudi, CEM, K562, HPB.ALL, Jurkat, Raji, and human lymphocytes. Human lymph node and tonsil.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Kishimoto T. et al., eds. Leukocyte Typing VI, p521-522 and p1157, Garland Publishing, Inc, New York and London, 1997. Shichishima T. et al. Diagnosis of paroxysmal nocturnal haemoglobinuria by phenotypic analysis of erythrocytes using two-colour flow cytometry with monoclonal antibodies to DAF and CD59/MACIF. Br J Haematol 1993, 85(2):378-386 Navenot JM. et al. Investigation of the survival of paroxysmal nocturnal hemoglobinuria red cells through the immunophenotyping of reticulocytes. Transfusion 1998 38(4):337-342. Murray EW and Robbins SM. Antibody cross-linking of the glycosylphosphatidylinositol-linked protein CD59 on hematopoietic cells induces signaling pathways resembling activation by complement. J Biol Chem 1998, 273(39):25279-25284

NCBI and Uniprot Product Information

• NCBI GI #: 10835165
• NCBI GeneID: 966
• NCBI Accession #: NP_000602.1
• NCBI GenBank Nucleotide #: NM_000611.5
• UniProt Accession #: P13987
• Molecular Weight: 20kDa
• NCBI Official Full Name: CD59 glycoprotein preproprotein
• NCBI Official Synonym Full Names: CD59 molecule
• NCBI Official Symbol: CD59
• NCBI Official Synonym Symbols: 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
• NCBI Protein Information: CD59 glycoprotein
• UniProt Protein Name: CD59 glycoprotein
• Protein Family: CD59 glycoprotein
• UniProt Gene Name: CD59
• UniProt Synonym Gene Names: MIC11; MIN1; MIN2; MIN3; MSK21; HRF-20; HRF20; MAC-IP; MACIF; MIRL
• UniProt Entry Name: CD59_HUMAN

NCBI Description

This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CD59: Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: anchored to external side of plasma membrane; cell surface; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; extracellular space; focal adhesion; Golgi membrane; membrane; plasma membrane; vesicle

Molecular Function: complement binding; protein binding

Biological Process: blood coagulation; cell activation; cell surface receptor linked signal transduction; COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; regulation of complement activation

Disease: Hemolytic Anemia, Cd59-mediated, With Or Without Immune-mediated Polyneuropathy