Mouse CD58 Monoclonal Antibody | anti-CD58 antibody

MOUSE ANTI HUMAN CD58:APC

Cat. Num. AAA215530

• Gene Names: CD58; ag3; LFA3; LFA-3
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD58; Monoclonal Antibody; MOUSE ANTI HUMAN CD58:APC; anti-CD58 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: MEM-63
• Specificity: CD58, or LFA-3, is a membrane glycoprotein of 55-70kD. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. It is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes[1]. It is a member of the immunoglobulin superfamily of molecules.CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells[2]. There are approximately 5,000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria.
• Form/Format: APC; Purified IgG conjugated to Allophycocyanin (APC) - lyophilised
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)
• Sequence Length: 248

• Applicable Applications for anti-CD58 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10
• Reconstitution: Reconstitute with 1ml distilled water ; Perservative Stabilisers
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of reconstitution.

Testing Data #1

(Staining of human peripheral blood granulocytes with Mouse anti Human CD58:APC (MBS215530))

Testing Data #2

(Staining of human peripheral blood granulocytes with Mouse anti Human CD58:RPE (MBS213729))

Testing Data #3

(Staining of human peripheral blood monocytes with Mouse anti Human CD58 (MBS212515))

Testing Data #4

(Staining of human peripheral blood monocytes with Mouse anti Human CD58: Azide Free (MBS210212))

Testing Data #6

(Staining of human peripheral blood platelets with Mouse anti Human CD58:FITC (MBS210848))

NCBI and Uniprot Product Information

• NCBI GI #: 221316575
• NCBI GeneID: 965
• NCBI Accession #: NP_001138294.1
• NCBI GenBank Nucleotide #: NM_001144822.1
• Molecular Weight: 27,960 Da
• NCBI Official Full Name: lymphocyte function-associated antigen 3 isoform 2
• NCBI Official Synonym Full Names: CD58 molecule
• NCBI Official Symbol: CD58
• NCBI Official Synonym Symbols: ag3; LFA3; LFA-3
• NCBI Protein Information: lymphocyte function-associated antigen 3; CD58 antigen, (lymphocyte function-associated antigen 3); surface glycoprotein LFA-3
• UniProt Protein Name: Lymphocyte function-associated antigen 3
• Protein Family: Lymphocyte function-associated antigen
• UniProt Gene Name: CD58
• UniProt Synonym Gene Names: LFA3; Ag3
• UniProt Entry Name: LFA3_HUMAN

NCBI Description

This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]

Uniprot Description

CD58: Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen- presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, GPI anchor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: cell surface; membrane; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: heterotypic cell-cell adhesion; cell-cell adhesion; cell adhesion; blood coagulation; leukocyte migration