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Mouse CD58 Monoclonal Antibody | anti-CD58 antibody

MOUSE ANTI HUMAN CD58

Gene Names
CD58; ag3; LFA3; LFA-3
Applications
Flow Cytometry, Functional Assay, Western Blot
Synonyms
CD58; Monoclonal Antibody; MOUSE ANTI HUMAN CD58; anti-CD58 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
BRIC5
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
248
Applicable Applications for anti-CD58 antibody
Flow cytometry (FC/FACS), Western Blot (WB)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: 1/10
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Related Product Information for anti-CD58 antibody
Mouse anti Human CD58 antibody, clone BRIC5 recognizes human Lymphocyte function-associated antigen 3, also known as CD58 or LFA-3. CD58 is a 250 amino acid single pass type I transmembrane glycoprotein, a member of the immunoglobulin superfamily, with a predicted molecular mass of 28.1 kDa and an apparent molecular mass of ~55-70 kDa. CD58 occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA and is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes (Makgoba et al. 1989).CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells. There are approximately 5,000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria.Mouse anti Human CD58 antibody, clone BRIC5 was produced in response to erythrocytes. The functional affinity of BRIC5 binding to erythrocytes is 4 x 108 M-1. It reacts by immunoblotting to non-reduced erythrocyte membranes. BRIC5 is an indirect haemagglutinin. The antigen on erythrocytes is pronase sensitive. BRIC5 inhibits T cell rosetting.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
965
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,960 Da
NCBI Official Full Name
lymphocyte function-associated antigen 3 isoform 2
NCBI Official Synonym Full Names
CD58 molecule
NCBI Official Symbol
CD58
NCBI Official Synonym Symbols
ag3; LFA3; LFA-3
NCBI Protein Information
lymphocyte function-associated antigen 3; CD58 antigen, (lymphocyte function-associated antigen 3); surface glycoprotein LFA-3
UniProt Protein Name
Lymphocyte function-associated antigen 3
UniProt Gene Name
CD58
UniProt Synonym Gene Names
LFA3; Ag3
UniProt Entry Name
LFA3_HUMAN

NCBI Description

This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]

Uniprot Description

CD58: Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen- presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: cell surface; membrane; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: heterotypic cell-cell adhesion; cell-cell adhesion; cell adhesion; blood coagulation; leukocyte migration

Research Articles on CD58

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Product Notes

The CD58 cd58 (Catalog #AAA213199) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD58 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS), Western Blot (WB). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Maximum Dilution: 1/10. Researchers should empirically determine the suitability of the CD58 cd58 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD58, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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