ELISA (EIA)
(Binding curve of anti-CD56 antibody NCAM12.19 (MBS488697) to recombinant human NCAM1 Fc-Fusion Protein. ELISA Plate coated with recombinant human NCAM1 Fc-Fusion Protein at a concentration of 5ug/ml. A 3-fold serial dilution from 10,000 ng/ml was performed using MBS488697. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.)
Mouse anti-Human CD56 Monoclonal Antibody | anti-CD56 antibody
Anti-CD56 [NCAM12.19]
ELISA (EIA)
(Binding curve of anti-CD56 antibody NCAM12.19 (MBS488697) to recombinant human NCAM1 Fc-Fusion Protein. ELISA Plate coated with recombinant human NCAM1 Fc-Fusion Protein at a concentration of 5ug/ml. A 3-fold serial dilution from 10,000 ng/ml was performed using MBS488697. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.)
ELISA (EIA)
(Binding curve of anti-CD56 antibody NCAM12.19 (MBS488697) to recombinant human NCAM1 Fc-Fusion Protein. ELISA Plate coated with recombinant human NCAM1 Fc-Fusion Protein at a concentration of 5ug/ml. A 3-fold serial dilution from 10,000 ng/ml was performed using MBS488697. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.)
Western Blot (WB)
(Western Blot using anti-CD56 antibody NCAM12.19 (MBS488697). human brain cerebellum(A) and cerebral cortex(B) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of NCAM12.19 () at 0.03ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Western Blot (WB)
(Western Blot using anti-CD56 antibody NCAM12.19 (MBS488697). human brain cerebellum(A) and cerebral cortex(B) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of NCAM12.19 () at 0.03ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Immunofluorescence (IF)
(Immunofluorescence staining of Kelly cells using anti-CD56 (MBS488697) NCAM12.19 Immunofluorescence analysis of paraformaldehyde fixed Kelly cells stained with the chimeric mouse IgG version of NCAM12.19 () at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488526, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (MBS488140) followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of Kelly cells using anti-CD56 (MBS488697) NCAM12.19 Immunofluorescence analysis of paraformaldehyde fixed Kelly cells stained with the chimeric mouse IgG version of NCAM12.19 () at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488526, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (MBS488140) followed by Alexa Fluor 488 secondary antibody.)
Western Blot (WB)
(Western Blot using anti-CD56 antibody NCAM12.19 (MBS488697). human brain cerebellum(A) and cerebral cortex(B) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of NCAM12.19 () at 0.03ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Western Blot (WB)
(Western Blot using anti-CD56 antibody NCAM12.19 (MBS488697). human brain cerebellum(A) and cerebral cortex(B) tissue lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of NCAM12.19 () at 0.03ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
This antibody was raised against human NCAM/CD56
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]
Uniprot Description
NCAM1: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, GPI anchor; Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 11q23.1
Cellular Component: Golgi membrane; cell surface; membrane; integral to membrane; plasma membrane; external side of plasma membrane
Molecular Function: identical protein binding
Biological Process: axon guidance; extracellular matrix organization and biogenesis; cytokine and chemokine mediated signaling pathway; cell adhesion
Research Articles on CD56
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Product Notes
The CD56 ncam1 (Catalog #AAA488696) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD56 [NCAM12.19] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD56 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA (EIA). Researchers should empirically determine the suitability of the CD56 ncam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD56, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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