Mouse CD55 Monoclonal Antibody | anti-CD55 antibody

MOUSE ANTI HUMAN CD55

Cat. Num. AAA212242

• Gene Names: CD55; CR; TC; DAF; CROM
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
• Synonyms: CD55; Monoclonal Antibody; MOUSE ANTI HUMAN CD55; anti-CD55 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 67
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 551

• Applicable Applications for anti-CD55 antibody: Immunohistology Frozen, Flow cytometry (FC/FACS), Western Blot (WB)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Please note: MyBioSource does not recommend the use of this reagent to stain erythrocytes; Immunohistology - Frozen: Minimum Dilution: 1/100; Maximum Dilution: 1/1000; Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/25
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Immunogen: K562 cells; Histology Positive Control Tissue
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of human peripheral blood monocytes with Mouse anti Human CD55)

Testing Data

(Staining of human peripheral blood monocytes with Mouse anti Human CD55:RPE (MBS213646))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD55 (MBS212243))

Related Product Information for anti-CD55 antibody

Mouse anti Human CD55 antibody, clone 67 recognizes the human CD55 cell surface antigen, a GPI linked molecule also known as decay accelerating factor (DAF). CD55 is expressed by a wide range of cell types. Erythrocyte expression is very weak. CD55 is the complement regulatory protein, decay accelerating factor (DAF) (Lublin and Atkinson 1989). Human CD55 is a ~70kDa glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans, and also on N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF carries the Cromer related blood group antigens. DAF has a wide distribution on cells in non-haemopoietic tissues, particularly epithelium and is specifically found at the foetal-maternal interface in placenta (Holmes et al. 1990 and Yang et al. 2009). Soluble forms of DAF are found, for example, in plasma, saliva and urine. The antigen on erythrocytes is pronase and chymotrypsin sensitive, but resistant to trypsin.

NCBI and Uniprot Product Information

• NCBI GI #: 10835143
• NCBI GeneID: 1604
• NCBI Accession #: NP_000565.1
• NCBI GenBank Nucleotide #: NM_000574.4
• UniProt Accession #: P08174; P09679; P78361; Q14UF2; Q14UF3; Q14UF4; Q14UF5; Q14UF6; B1AP14; D3DT83; D3DT84; E7ER69
• Molecular Weight: 59,038 Da
• NCBI Official Full Name: complement decay-accelerating factor isoform 1 preproprotein
• NCBI Official Synonym Full Names: CD55 molecule, decay accelerating factor for complement (Cromer blood group)
• NCBI Official Symbol: CD55
• NCBI Official Synonym Symbols: CR; TC; DAF; CROM
• NCBI Protein Information: complement decay-accelerating factor; CD55 antigen
• UniProt Protein Name: Complement decay-accelerating factor
• Protein Family: Complement decay-accelerating factor
• UniProt Gene Name: CD55
• UniProt Synonym Gene Names: CR; DAF
• UniProt Entry Name: DAF_HUMAN

NCBI Description

This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014]

Uniprot Description

CD55: This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Belongs to the receptors of complement activation (RCA) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: cell surface; integral to plasma membrane; plasma membrane; extracellular region; lipid raft

Molecular Function: viral receptor activity; protein binding; lipid binding

Biological Process: respiratory burst; entry of virus into host cell; elevation of cytosolic calcium ion concentration; negative regulation of complement activation; regulation of complement activation; innate immune response; complement activation, classical pathway; regulation of lipopolysaccharide-mediated signaling pathway

Disease: Blood Group, Cromer System