Mouse anti-Human CD52 Monoclonal Antibody | anti-CD52 antibody

Anti-Hu CD52 FITC

Cat. Num. AAA1801906

• Gene Names: CD52; CDW52; EDDM5
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD52; Monoclonal Antibody; Anti-Hu CD52 FITC; CAMPATH-1; HE5; ESPE5; CD52 molecule; anti-CD52 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG3
• Clone Number: 4C8
• Specificity: The mouse monoclonal antibody 4C8 recognizes CD52, a GPI-anchored glycopeptide expressed on the surface of lymphocytes, macrophages, epithelial cells of male reproductive tract, and mature sperms.
• Purity/Purification: The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD52 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Conjugation: FITC
• Immunogen: human lymphocytes
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD52 (4C8) FITC antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD52 (4C8) FITC antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Related Product Information for anti-CD52 antibody

CD52 (CAMPATH-1, HE5) is a highly glycosylated GPI-anchored 21-28kDa glycopeptide which is present at high levels on lymphocytes, macrophages, epithelial cells of male reproductive tract and mature sperm. Its 12-amino acid beckbone carries a complex N-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well as the GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where it is released by epithelial cells. Although CD52 is not an essential T-cell costimulator, its triggering results in activation of normal human T cells. CD52 is a very good target for antibody/complement-mediated cell lysis.

References

*Masuyama J, Kaga S, Kano S, Minota S: A novel costimulation pathway via the 4C8 antigen for the induction of CD4+ regulatory T cells. J Immunol. 2002 Oct 1;169(7):3710-6., URL: https://pubmed.ncbi.nlm.nih.gov/12244164/from_term=masuyama+kaga+&from_pos=4,*Masuyama J, Yoshio T, Suzuki K, Kitagawa S, Iwamoto M, Kamimura T, Hirata D, Takeda A, Kano S, Minota S: Characterization of the 4C8 antigen involved in transendothelial migration of CD26(hi) T cells after tight adhesion to human umbilical vein endothelial cell monolayers. J Exp Med. 1999 Mar 15;189(6):979-90., URL: https://pubmed.ncbi.nlm.nih.gov/10075981/from_term=masuyama+yoshio+suzuki&from_schema=all&from_pos=3,*Toh BH, Kyaw T, Tipping P, Bobik A: Immune Regulation by CD52-expressing CD4 T Cells. Cell Mol Immunol. 2013 Sep;10(5):379-82., URL: https://pubmed.ncbi.nlm.nih.gov/23934027/from_term=tipping+p+bobik+a+kyaw&from_page=2&from_pos=6,*Watanabe T, Masuyama J, Sohma Y, Inazawa H, Horie K, Kojima K, Uemura Y, Aoki Y, Kaga S, Minota S, Tanaka T, Yamaguchi Y, Kobayashi T, Serizawa I: CD52 is a novel costimulatory molecule for induction of CD4+ regulatory T cells. Clin Immunol. 2006 Sep;120(3):247-59., URL: https://pubmed.ncbi.nlm.nih.gov/16797237/from_term=watanabe+inazawa&from_page=2&from_pos=7
*Schröter S, Derr P, Conradt HS, Nimtz M, Hale G, Kirchhoff C: Male-specific modification of human CD52. J Biol Chem. 1999 Oct 15;274(42):29862-73., URL: https://www.ncbi.nlm.nih.gov/pubmed/10514467ordin,*Treumann A, Lifely MR, Schneider P, Ferguson MA: Primary structure of CD52. J Biol Chem. 1995 Mar 17;270(11):6088-99., URL: https://www.ncbi.nlm.nih.gov/pubmed/7890742ordina,*Rowan WC, Hale G, Tite JP, Brett SJ: Cross-linking of the CAMPATH-1 antigen (CD52) triggers activation of normal human T lymphocytes. Int Immunol. 1995 Jan;7(1):69-77., URL: https://www.ncbi.nlm.nih.gov/pubmed/7718516ordina,*Koyama K, Ito K, Hasegawa A: Role of male reproductive tract CD52 (mrt-CD52) in reproduction. Soc Reprod Fertil Suppl. 2007;63:103-10., URL: https://www.ncbi.nlm.nih.gov/pubmed/17566265ordin,*Domaga?a A, Kurpisz M: CD52 antigen--a review. Med Sci Monit. 2001 Mar-Apr;7(2):325-31., URL: https://www.ncbi.nlm.nih.gov/pubmed/11257744ordin

NCBI and Uniprot Product Information

• NCBI GI #: 399214
• NCBI GeneID: 1043
• UniProt Accession #: P31358; Q5T138; Q9BW46
• Molecular Weight: 6,614 Da
• NCBI Official Full Name: CAMPATH-1 antigen
• NCBI Official Synonym Full Names: CD52 molecule
• NCBI Official Symbol: CD52
• NCBI Official Synonym Symbols: CDW52; EDDM5
• NCBI Protein Information: CAMPATH-1 antigen
• UniProt Protein Name: CAMPATH-1 antigen
• Protein Family: CAMPATH-1 antigen
• UniProt Gene Name: CD52
• UniProt Synonym Gene Names: CDW52; HE5; He5

Uniprot Description

May play a role in carrying and orienting carbohydrate, as well as having a more specific role.