Mouse anti-Human CD5 Monoclonal Antibody | anti-CD5 antibody

Anti-Hu CD5 Biotin

Cat. Num. AAA1800838

• Gene Names: CD5; T1; LEU1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot, Immunocytochemistry
• Purity: The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Synonyms: CD5; Monoclonal Antibody; Anti-Hu CD5 Biotin; CD5 molecule; T1; LEU1; anti-CD5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a kappa
• Clone Number: L17F12
• Specificity: The mouse monoclonal antibody L17F12 reacts with an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
• Purity/Purification: The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 495

• Applicable Applications for anti-CD5 antibody: Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: FC/FACS: QC tested
• Immunogen: Human acute lymphoblastic leukemia (ALL) T cells
• Conjugation: Biotin
• Preparation and Storage: Store at 2-8 degree C.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of CD5 in human peripheral blood cells with anti-CD5 (L17F12) biotin.)

Related Product Information for anti-CD5 antibody

CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains.The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies.Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.

NCBI and Uniprot Product Information

• NCBI GI #: 166197668
• NCBI GeneID: 921
• NCBI Accession #: NP_055022.2
• NCBI GenBank Nucleotide #: NP_055022.2
• UniProt Accession #: P06127
• NCBI Official Full Name: T-cell surface glycoprotein CD5 isoform 1
• NCBI Official Synonym Full Names: CD5 molecule
• NCBI Official Symbol: CD5
• NCBI Official Synonym Symbols: T1; LEU1
• NCBI Protein Information: T-cell surface glycoprotein CD5
• UniProt Protein Name: T-cell surface glycoprotein CD5
• Protein Family: CD5 antigen
• UniProt Gene Name: CD5
• UniProt Synonym Gene Names: LEU1
• UniProt Entry Name: CD5_HUMAN

NCBI Description

This gene encodes a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2016]

Uniprot Description

CD5: a surface glycoprotein expressed on T cells and B-1 B cells. Rapidly recruited to the immunological synapse, lowering the response of the T cell antigen receptor. CD5 interacts with CD72/LYB-2. Promotes B-cell survival through stimulation of autocrine IL-10 production. Contains 3 SRCR domains.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor activity; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; cell proliferation; T cell costimulation; cell recognition